Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 59004..59740 Replicon plasmid p309074-1
Accession NZ_CP030297
Organism Klebsiella pneumoniae strain 309074

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag DQG43_RS26085 Protein ID WP_003026803.1
Coordinates 59258..59740 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag DQG43_RS26080 Protein ID WP_003026799.1
Coordinates 59004..59270 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DQG43_RS26035 (DQG43_26830) 55066..55428 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
DQG43_RS26040 (DQG43_26835) 55478..55828 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
DQG43_RS26045 (DQG43_26840) 56186..56455 + 270 WP_004152102.1 hypothetical protein -
DQG43_RS26050 (DQG43_26845) 56443..57018 + 576 WP_004152103.1 hypothetical protein -
DQG43_RS26055 (DQG43_26850) 57049..57543 + 495 WP_004152104.1 DNA-binding protein -
DQG43_RS26060 (DQG43_26855) 57587..57955 + 369 WP_004152105.1 hypothetical protein -
DQG43_RS26065 (DQG43_26860) 57989..58192 + 204 WP_004152106.1 HHA domain-containing protein -
DQG43_RS26070 (DQG43_26865) 58241..58498 + 258 WP_004152107.1 hypothetical protein -
DQG43_RS26075 (DQG43_26870) 58574..58828 + 255 WP_004152108.1 hypothetical protein -
DQG43_RS26080 (DQG43_26875) 59004..59270 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
DQG43_RS26085 (DQG43_26880) 59258..59740 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
DQG43_RS26090 (DQG43_26885) 59952..61298 + 1347 WP_020314316.1 ISNCY family transposase -
DQG43_RS27860 61459..61590 + 132 WP_004218042.1 hypothetical protein -
DQG43_RS26095 (DQG43_26890) 61799..62964 - 1166 Protein_66 IS3 family transposase -
DQG43_RS26100 (DQG43_26895) 63141..64103 - 963 WP_004152113.1 zinc metalloprotease HtpX -
DQG43_RS26105 (DQG43_26900) 64090..64578 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / tet(A) / dfrA12 / aadA2 / qacE / sul1 / qnrA1 / floR / tet(G) / rmtB / blaTEM-1B - 1..185726 185726


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T107529 WP_003026803.1 NZ_CP030297:59258-59740 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T107529 NZ_CP030297:59258-59740 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT107529 WP_003026799.1 NZ_CP030297:59004-59270 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT107529 NZ_CP030297:59004-59270 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References