Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5234261..5234886 Replicon chromosome
Accession NZ_CP030296
Organism Klebsiella pneumoniae strain 309074

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag DQG43_RS25410 Protein ID WP_019705794.1
Coordinates 5234261..5234644 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag DQG43_RS25415 Protein ID WP_004150355.1
Coordinates 5234644..5234886 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DQG43_RS25395 (5231627) 5231627..5232529 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
DQG43_RS25400 (5232526) 5232526..5233161 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
DQG43_RS25405 (5233158) 5233158..5234087 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
DQG43_RS25410 (5234261) 5234261..5234644 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
DQG43_RS25415 (5234644) 5234644..5234886 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
DQG43_RS25420 (5235091) 5235091..5236008 + 918 WP_004146235.1 alpha/beta hydrolase -
DQG43_RS25425 (5236023) 5236023..5236964 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
DQG43_RS25430 (5237009) 5237009..5237446 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
DQG43_RS25435 (5237443) 5237443..5238303 - 861 WP_004146232.1 virulence factor BrkB family protein -
DQG43_RS25440 (5238297) 5238297..5238896 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T107528 WP_019705794.1 NZ_CP030296:c5234644-5234261 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T107528 NZ_CP030296:c5234644-5234261 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT107528 WP_004150355.1 NZ_CP030296:c5234886-5234644 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT107528 NZ_CP030296:c5234886-5234644 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References