Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3996268..3996887 Replicon chromosome
Accession NZ_CP030296
Organism Klebsiella pneumoniae strain 309074

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag DQG43_RS19595 Protein ID WP_002892050.1
Coordinates 3996669..3996887 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag DQG43_RS19590 Protein ID WP_002892066.1
Coordinates 3996268..3996642 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DQG43_RS19580 (3991420) 3991420..3992613 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
DQG43_RS19585 (3992636) 3992636..3995782 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
DQG43_RS19590 (3996268) 3996268..3996642 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
DQG43_RS19595 (3996669) 3996669..3996887 + 219 WP_002892050.1 HHA domain-containing protein Toxin
DQG43_RS19600 (3997050) 3997050..3997616 + 567 WP_002892030.1 maltose O-acetyltransferase -
DQG43_RS19605 (3997588) 3997588..3997728 - 141 WP_004147370.1 hypothetical protein -
DQG43_RS19610 (3997749) 3997749..3998219 + 471 WP_002892026.1 YlaC family protein -
DQG43_RS19615 (3998194) 3998194..3999645 - 1452 WP_064151794.1 PLP-dependent aminotransferase family protein -
DQG43_RS19620 (3999746) 3999746..4000444 + 699 WP_002892021.1 GNAT family protein -
DQG43_RS19625 (4000441) 4000441..4000581 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
DQG43_RS19630 (4000581) 4000581..4000844 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T107524 WP_002892050.1 NZ_CP030296:3996669-3996887 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T107524 NZ_CP030296:3996669-3996887 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT107524 WP_002892066.1 NZ_CP030296:3996268-3996642 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT107524 NZ_CP030296:3996268-3996642 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References