Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 159960..160603 Replicon plasmid p721005-2
Accession NZ_CP030294
Organism Klebsiella pneumoniae strain 721005

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag DRB00_RS29330 Protein ID WP_001044770.1
Coordinates 159960..160376 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag DRB00_RS29335 Protein ID WP_001261282.1
Coordinates 160373..160603 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRB00_RS29310 (DRB00_30505) 156063..156335 - 273 Protein_192 transposase -
DRB00_RS29320 (DRB00_30515) 157317..158339 - 1023 WP_000361404.1 helicase UvrD -
DRB00_RS29325 (DRB00_30520) 158324..159886 - 1563 WP_004206609.1 AAA family ATPase -
DRB00_RS29330 (DRB00_30525) 159960..160376 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
DRB00_RS29335 (DRB00_30530) 160373..160603 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
DRB00_RS29340 (DRB00_30535) 160560..161021 + 462 WP_074193041.1 hypothetical protein -
DRB00_RS29345 (DRB00_30540) 161165..161515 + 351 WP_000493378.1 hypothetical protein -
DRB00_RS29350 (DRB00_30545) 161566..162309 + 744 WP_000129823.1 hypothetical protein -
DRB00_RS29355 (DRB00_30550) 162306..163082 + 777 WP_000015958.1 site-specific integrase -
DRB00_RS29360 (DRB00_30555) 163140..163397 - 258 WP_000764642.1 hypothetical protein -
DRB00_RS29365 163526..163630 - 105 WP_032409716.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA2 / blaTEM-1C / dfrA25 / qacE / sul1 / qnrB52 / blaKPC-2 - 1..164198 164198


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T107511 WP_001044770.1 NZ_CP030294:c160376-159960 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T107511 NZ_CP030294:c160376-159960 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT107511 WP_001261282.1 NZ_CP030294:c160603-160373 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT107511 NZ_CP030294:c160603-160373 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References