Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 19087..19730 | Replicon | plasmid p721005-2 |
| Accession | NZ_CP030294 | ||
| Organism | Klebsiella pneumoniae strain 721005 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | Q84A06 |
| Locus tag | DRB00_RS28470 | Protein ID | WP_000754566.1 |
| Coordinates | 19314..19730 (+) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | Q84A07 |
| Locus tag | DRB00_RS28465 | Protein ID | WP_001261276.1 |
| Coordinates | 19087..19317 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| DRB00_RS28435 (DRB00_29495) | 15219..15923 | + | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| DRB00_RS28440 (DRB00_29500) | 16030..17046 | - | 1017 | WP_017899884.1 | hypothetical protein | - |
| DRB00_RS28445 (DRB00_29505) | 17057..17371 | - | 315 | WP_053389906.1 | hypothetical protein | - |
| DRB00_RS28450 (DRB00_29510) | 17398..17793 | - | 396 | WP_017899885.1 | hypothetical protein | - |
| DRB00_RS28455 (DRB00_29515) | 17962..18267 | - | 306 | WP_013023785.1 | type II toxin-antitoxin system toxin CcdB | - |
| DRB00_RS28460 (DRB00_29520) | 18269..18487 | - | 219 | WP_001568025.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| DRB00_RS28465 (DRB00_29535) | 19087..19317 | + | 231 | WP_001261276.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| DRB00_RS28470 (DRB00_29540) | 19314..19730 | + | 417 | WP_000754566.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| DRB00_RS28475 (DRB00_29545) | 19934..20790 | + | 857 | Protein_21 | IS3-like element ISEc15 family transposase | - |
| DRB00_RS28480 (DRB00_29555) | 21072..22148 | + | 1077 | WP_000227969.1 | IS110 family transposase | - |
| DRB00_RS28485 | 22710..22856 | - | 147 | WP_032072915.1 | hypothetical protein | - |
| DRB00_RS28490 (DRB00_29575) | 22995..23843 | + | 849 | WP_032072916.1 | hypothetical protein | - |
| DRB00_RS28495 (DRB00_29580) | 23903..24187 | + | 285 | WP_058676404.1 | hypothetical protein | - |
| DRB00_RS28500 (DRB00_29585) | 24276..24569 | + | 294 | WP_032072918.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | catA2 / blaTEM-1C / dfrA25 / qacE / sul1 / qnrB52 / blaKPC-2 | - | 1..164198 | 164198 | |
| - | inside | IScluster/Tn | - | - | 7891..26585 | 18694 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15006.52 Da Isoelectric Point: 9.2957
>T107510 WP_000754566.1 NZ_CP030294:19314-19730 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK
Download Length: 417 bp
>T107510 NZ_CP030294:19314-19730 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8721.74 Da Isoelectric Point: 4.3533
>AT107510 WP_001261276.1 NZ_CP030294:19087-19317 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP
Download Length: 231 bp
>AT107510 NZ_CP030294:19087-19317 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A387K1G3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A387K023 |