Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 17962..18487 Replicon plasmid p721005-2
Accession NZ_CP030294
Organism Klebsiella pneumoniae strain 721005

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag DRB00_RS28455 Protein ID WP_013023785.1
Coordinates 17962..18267 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag DRB00_RS28460 Protein ID WP_001568025.1
Coordinates 18269..18487 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRB00_RS28435 (DRB00_29495) 15219..15923 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
DRB00_RS28440 (DRB00_29500) 16030..17046 - 1017 WP_017899884.1 hypothetical protein -
DRB00_RS28445 (DRB00_29505) 17057..17371 - 315 WP_053389906.1 hypothetical protein -
DRB00_RS28450 (DRB00_29510) 17398..17793 - 396 WP_017899885.1 hypothetical protein -
DRB00_RS28455 (DRB00_29515) 17962..18267 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
DRB00_RS28460 (DRB00_29520) 18269..18487 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
DRB00_RS28465 (DRB00_29535) 19087..19317 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin -
DRB00_RS28470 (DRB00_29540) 19314..19730 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin -
DRB00_RS28475 (DRB00_29545) 19934..20790 + 857 Protein_21 IS3-like element ISEc15 family transposase -
DRB00_RS28480 (DRB00_29555) 21072..22148 + 1077 WP_000227969.1 IS110 family transposase -
DRB00_RS28485 22710..22856 - 147 WP_032072915.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA2 / blaTEM-1C / dfrA25 / qacE / sul1 / qnrB52 / blaKPC-2 - 1..164198 164198
- inside IScluster/Tn - - 7891..26585 18694


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T107509 WP_013023785.1 NZ_CP030294:c18267-17962 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T107509 NZ_CP030294:c18267-17962 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT107509 WP_001568025.1 NZ_CP030294:c18487-18269 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT107509 NZ_CP030294:c18487-18269 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References