107508

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 190254..190776 Replicon plasmid p721005-1
Accession NZ_CP030293
Organism Klebsiella pneumoniae strain 721005

Toxin (Protein)


Gene name stbE Uniprot ID A0A2J4R0S6
Locus tag DRB00_RS28005 Protein ID WP_004181778.1
Coordinates 190492..190776 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag DRB00_RS28000 Protein ID WP_004181777.1
Coordinates 190254..190502 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRB00_RS27980 (DRB00_28980) 185463..186284 - 822 WP_004181772.1 hypothetical protein -
DRB00_RS27985 (DRB00_28985) 186346..186699 - 354 WP_004181774.1 hypothetical protein -
DRB00_RS27990 (DRB00_28990) 186844..187830 - 987 WP_101992392.1 hypothetical protein -
DRB00_RS27995 (DRB00_28995) 188164..189963 - 1800 WP_004181776.1 ATP-dependent helicase -
DRB00_RS28000 (DRB00_29000) 190254..190502 + 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
DRB00_RS28005 (DRB00_29005) 190492..190776 + 285 WP_004181778.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DRB00_RS28010 (DRB00_29010) 190793..190894 - 102 Protein_205 IS200/IS605 family transposase -
DRB00_RS28015 (DRB00_29015) 190930..192156 + 1227 WP_040209639.1 RNA-guided endonuclease TnpB family protein -
DRB00_RS28020 (DRB00_29020) 192427..192651 - 225 Protein_207 transposase -
DRB00_RS28025 (DRB00_29025) 192730..193158 - 429 WP_077257669.1 IS200/IS605 family transposase -
DRB00_RS28030 (DRB00_29030) 193194..194381 + 1188 WP_040209642.1 RNA-guided endonuclease TnpB family protein -
DRB00_RS29985 194426..194797 - 372 WP_040209644.1 hypothetical protein -
DRB00_RS29990 194794..195138 - 345 WP_223811496.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - iucA / iucB / iucC / iucD / iutA / rmpA 1..269204 269204


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10970.69 Da        Isoelectric Point: 10.6516

>T107508 WP_004181778.1 NZ_CP030293:190492-190776 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Download         Length: 285 bp

>T107508 NZ_CP030293:190492-190776 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT107508 WP_004181777.1 NZ_CP030293:190254-190502 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT107508 NZ_CP030293:190254-190502 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R0S6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References