Detailed information of TA system TA107507

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 116957..117627
• Replicon: plasmid p721005-1
• Accession: NZ_CP030293
• Organism: Klebsiella pneumoniae strain 721005

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: DRB00_RS27620
• Protein ID: WP_004213072.1
• Coordinates: 116957..117400 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: DRB00_RS27625
• Protein ID: WP_004213073.1
• Coordinates: 117397..117627 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DRB00_RS27585 (DRB00_28530)	112369..112644	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
DRB00_RS27590 (DRB00_28535)	112707..113198	+	492	WP_011251327.1	DM13 domain-containing protein	-
DRB00_RS27595 (DRB00_28540)	113247..114167	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
DRB00_RS27600 (DRB00_28550)	114258..114661	+	404	Protein_124	GAF domain-containing protein	-
DRB00_RS27605	115179..115813	-	635	Protein_125	mucoid phenotype regulator RmpA2	-
DRB00_RS27610 (DRB00_28570)	116230..116534	+	305	Protein_126	transposase	-
DRB00_RS29980	116557..116808	-	252	WP_186987481.1	hypothetical protein	-
DRB00_RS27620 (DRB00_28580)	116957..117400	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
DRB00_RS27625 (DRB00_28585)	117397..117627	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
DRB00_RS27630 (DRB00_28590)	118235..119368	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
DRB00_RS27635 (DRB00_28595)	119384..119677	+	294	WP_004213076.1	hypothetical protein	-
DRB00_RS27640 (DRB00_28600)	119667..119873	-	207	WP_004213077.1	hypothetical protein	-
DRB00_RS27645 (DRB00_28605)	120225..120515	+	291	WP_004213078.1	hypothetical protein	-
DRB00_RS27650 (DRB00_28610)	120505..121404	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA	1..269204	269204

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T107507 WP_004213072.1 NZ_CP030293:c117400-116957 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T107507 NZ_CP030293:c117400-116957 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT107507 WP_004213073.1 NZ_CP030293:c117627-117397 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT107507 NZ_CP030293:c117627-117397 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620