Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 41189..41715 Replicon plasmid p721005-1
Accession NZ_CP030293
Organism Klebsiella pneumoniae strain 721005

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag DRB00_RS27235 Protein ID WP_000323025.1
Coordinates 41428..41715 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag DRB00_RS27230 Protein ID WP_000534858.1
Coordinates 41189..41428 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRB00_RS27210 (DRB00_28105) 37187..38527 + 1341 WP_000589001.1 ISNCY family transposase -
DRB00_RS27215 (DRB00_28110) 38948..39259 + 312 Protein_45 transposase -
DRB00_RS27225 (DRB00_28125) 40510..40926 + 417 Protein_47 cation-efflux pump -
DRB00_RS29965 41060..41164 - 105 Protein_48 hypothetical protein -
DRB00_RS27230 (DRB00_28135) 41189..41428 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
DRB00_RS27235 (DRB00_28140) 41428..41715 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
DRB00_RS27240 (DRB00_28145) 41787..41945 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
DRB00_RS27245 42548..43522 + 975 WP_004181899.1 hypothetical protein -
DRB00_RS27250 (DRB00_28160) 43720..44097 - 378 WP_004181901.1 hypothetical protein -
DRB00_RS27255 (DRB00_28175) 44787..45854 + 1068 WP_004181903.1 hypothetical protein -
DRB00_RS27260 (DRB00_28180) 46010..46357 + 348 WP_004181904.1 hypothetical protein -
DRB00_RS27265 (DRB00_28185) 46436..46669 + 234 WP_004181905.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - iucA / iucB / iucC / iucD / iutA / rmpA 1..269204 269204


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T107506 WP_000323025.1 NZ_CP030293:41428-41715 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T107506 NZ_CP030293:41428-41715 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT107506 WP_000534858.1 NZ_CP030293:41189-41428 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT107506 NZ_CP030293:41189-41428 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References