Detailed information of TA system TA107505

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 34007..34917
• Replicon: plasmid p721005-1
• Accession: NZ_CP030293
• Organism: Klebsiella pneumoniae strain 721005

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: DRB00_RS27195
• Protein ID: WP_004026354.1
• Coordinates: 34447..34917 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: DRB00_RS27190
• Protein ID: WP_004181895.1
• Coordinates: 34007..34450 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DRB00_RS27175 (DRB00_28065)	30640..32127	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
DRB00_RS27180 (DRB00_28075)	32213..32635	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
DRB00_RS27185 (DRB00_28080)	32635..33906	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
DRB00_RS27190 (DRB00_28085)	34007..34450	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
DRB00_RS27195 (DRB00_28090)	34447..34917	+	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
DRB00_RS27200 (DRB00_28095)	35027..35287	-	261	WP_004026352.1	hypothetical protein	-
DRB00_RS27205 (DRB00_28100)	35858..37039	-	1182	WP_078207741.1	IS481 family transposase	-
DRB00_RS27210 (DRB00_28105)	37187..38527	+	1341	WP_000589001.1	ISNCY family transposase	-
DRB00_RS27215 (DRB00_28110)	38948..39259	+	312	Protein_45	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA	1..269204	269204

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T107505 WP_004026354.1 NZ_CP030293:34447-34917 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T107505 NZ_CP030293:34447-34917 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT107505 WP_004181895.1 NZ_CP030293:34007-34450 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT107505 NZ_CP030293:34007-34450 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A2J4RLY9