Detailed information of TA system TA107485

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 45685..46220
• Replicon: plasmid pLKSZ04
• Accession: NZ_CP030285
• Organism: Escherichia coli strain E308

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: DQW89_RS27300
• Protein ID: WP_032206513.1
• Coordinates: 45933..46220 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: T1PXV2
• Locus tag: DQW89_RS27295
• Protein ID: WP_001139206.1
• Coordinates: 45685..45936 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQW89_RS27285	43071..45074	-	2004	Protein_57	helicase	-
DQW89_RS28020	45431..45598	+	168	WP_001352029.1	hypothetical protein	-
DQW89_RS27295	45685..45936	+	252	WP_001139206.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DQW89_RS27300	45933..46220	+	288	WP_032206513.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DQW89_RS27305	46510..46710	-	201	WP_000896262.1	DUF839 domain-containing protein	-
DQW89_RS27310	46700..46905	+	206	Protein_62	hypothetical protein	-
DQW89_RS27315	47080..50593	-	3514	Protein_63	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(A) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / blaTEM-1B	-	1..92978	92978

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11130.20 Da
• Isoelectric Point: 10.5833

>T107485 WP_032206513.1 NZ_CP030285:45933-46220 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQIIDDMLIIAVVAVGK
RERSNVYHLASERMK

Nucleotide

• Length: 288 bp

>T107485 NZ_CP030285:45933-46220 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGATTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9171.39 Da
• Isoelectric Point: 4.0927

>AT107485 WP_001139206.1 NZ_CP030285:45685-45936 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT107485 NZ_CP030285:45685-45936 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Antitoxin

• AlphaFold DB: T1PXV2