Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 6939..7540 Replicon plasmid pLKSZ04
Accession NZ_CP030285
Organism Escherichia coli strain E308

Toxin (Protein)


Gene name doc Uniprot ID F0JYA5
Locus tag DQW89_RS27035 Protein ID WP_001216044.1
Coordinates 7160..7540 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag DQW89_RS27030 Protein ID WP_001190712.1
Coordinates 6939..7160 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DQW89_RS26995 2801..2989 - 189 WP_000957857.1 hypothetical protein -
DQW89_RS27000 3235..3609 + 375 WP_039022008.1 hypothetical protein -
DQW89_RS27005 3591..4340 + 750 WP_171841598.1 hypothetical protein -
DQW89_RS27010 4461..4676 + 216 WP_024259737.1 hypothetical protein -
DQW89_RS28010 4850..4999 - 150 WP_162883679.1 hypothetical protein -
DQW89_RS27015 5469..5750 + 282 WP_118394096.1 DNA polymerase III subunit theta -
DQW89_RS27020 5728..6302 - 575 Protein_10 DNA-binding protein -
DQW89_RS27025 6477..6866 + 390 WP_000506730.1 DNA repair protein -
DQW89_RS27030 6939..7160 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
DQW89_RS27035 7160..7540 + 381 WP_001216044.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
DQW89_RS27040 7545..7724 + 180 WP_039022005.1 hypothetical protein -
DQW89_RS27045 7752..8621 + 870 Protein_15 DUF968 domain-containing protein -
DQW89_RS27050 8796..9647 - 852 WP_000611653.1 hypothetical protein -
DQW89_RS27055 9758..9967 - 210 WP_000874154.1 hypothetical protein -
DQW89_RS27070 10158..10379 + 222 Protein_18 IS1 family transposase -
DQW89_RS27075 10409..12160 - 1752 Protein_19 Tn3-like element TnAs1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / blaTEM-1B - 1..92978 92978


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13544.28 Da        Isoelectric Point: 5.6343

>T107484 WP_001216044.1 NZ_CP030285:7160-7540 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVAATLRRLYGSAE

Download         Length: 381 bp

>T107484 NZ_CP030285:7160-7540 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGCCACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT107484 WP_001190712.1 NZ_CP030285:6939-7160 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT107484 NZ_CP030285:6939-7160 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6Y6HMN5

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References