Detailed information of TA system TA107483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 58009..58531
• Replicon: plasmid pLKSZ03
• Accession: NZ_CP030284
• Organism: Escherichia coli strain E308

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: DQW89_RS26950
• Protein ID: WP_000638823.1
• Coordinates: 58250..58531 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: DQW89_RS26945
• Protein ID: WP_000121741.1
• Coordinates: 58009..58260 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQW89_RS26895	53098..53541	+	444	WP_000964330.1	NfeD family protein	-
DQW89_RS28000	53545..53715	+	171	WP_000550720.1	hypothetical protein	-
DQW89_RS26900	53726..54172	+	447	WP_001243165.1	hypothetical protein	-
DQW89_RS26905	54205..54462	+	258	WP_001542015.1	hypothetical protein	-
DQW89_RS26910	54443..54745	+	303	WP_001360345.1	hypothetical protein	-
DQW89_RS26915	54742..54999	+	258	WP_000739144.1	hypothetical protein	-
DQW89_RS26920	55003..55998	+	996	WP_001028543.1	type IV secretion system protein	-
DQW89_RS26925	56004..56645	+	642	WP_001425343.1	conjugal transfer protein TrbJ	-
DQW89_RS26930	56647..56901	+	255	WP_001043555.1	EexN family lipoprotein	-
DQW89_RS26935	56914..57201	+	288	WP_001032611.1	EexN family lipoprotein	-
DQW89_RS26940	57274..57909	+	636	WP_015059536.1	hypothetical protein	-
DQW89_RS26945	58009..58260	+	252	WP_000121741.1	hypothetical protein	Antitoxin
DQW89_RS26950	58250..58531	+	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DQW89_RS26955	58599..58898	+	300	WP_000835764.1	IncN plasmid KikA protein	-
DQW89_RS26960	58901..60137	+	1237	Protein_72	TcpQ domain-containing protein	-
DQW89_RS26965	60143..60580	+	438	WP_000539665.1	type IV pilus biogenesis protein PilM	-
DQW89_RS26970	60699..61097	+	399	WP_001153665.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mcr-1.1	-	1..61476	61476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T107483 WP_000638823.1 NZ_CP030284:58250-58531 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T107483 NZ_CP030284:58250-58531 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT107483 WP_000121741.1 NZ_CP030284:58009-58260 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT107483 NZ_CP030284:58009-58260 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1