Detailed information of TA system TA107482

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 42244..42745
• Replicon: plasmid pLKSZ03
• Accession: NZ_CP030284
• Organism: Escherichia coli strain E308

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: H1ZXU8
• Locus tag: DQW89_RS26800
• Protein ID: WP_000702247.1
• Coordinates: 42485..42745 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: DQW89_RS26795
• Protein ID: WP_001132407.1
• Coordinates: 42244..42495 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQW89_RS26745	37301..38053	+	753	WP_063078685.1	conjugal transfer protein TraL	-
DQW89_RS26750	38055..38762	+	708	WP_021503228.1	hypothetical protein	-
DQW89_RS26755	38782..39108	+	327	WP_063078684.1	hypothetical protein	-
DQW89_RS26760	39239..39676	-	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
DQW89_RS26765	39701..39883	-	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
DQW89_RS26770	40441..40623	+	183	WP_001481829.1	hypothetical protein	-
DQW89_RS26775	40730..40996	-	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
DQW89_RS26780	41416..41694	-	279	WP_000545938.1	hypothetical protein	-
DQW89_RS26795	42244..42495	-	252	WP_001132407.1	transcriptional regulator	Antitoxin
DQW89_RS26800	42485..42745	-	261	WP_000702247.1	hypothetical protein	Toxin
DQW89_RS26805	42797..43021	-	225	WP_000814381.1	hypothetical protein	-
DQW89_RS26810	43118..43327	-	210	WP_000866036.1	hypothetical protein	-
DQW89_RS26815	43368..43691	-	324	WP_000427403.1	hypothetical protein	-
DQW89_RS27990	44018..44164	+	147	WP_001356709.1	hypothetical protein	-
DQW89_RS26825	44273..44602	-	330	WP_000855120.1	hypothetical protein	-
DQW89_RS26835	45200..45865	+	666	WP_000532148.1	P-loop NTPase	-
DQW89_RS28150	45939..46223	+	285	WP_001162371.1	hypothetical protein	-
DQW89_RS26845	46241..46837	-	597	WP_000125165.1	hypothetical protein	-
DQW89_RS26850	46987..47148	-	162	WP_000775236.1	type I toxin-antitoxin system Hok family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mcr-1.1	-	1..61476	61476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9817.29 Da
• Isoelectric Point: 10.0665

>T107482 WP_000702247.1 NZ_CP030284:c42745-42485 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T107482 NZ_CP030284:c42745-42485 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT107482 WP_001132407.1 NZ_CP030284:c42495-42244 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT107482 NZ_CP030284:c42495-42244 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A1P8DNC0