Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 48519..49041 Replicon plasmid pLKSZ02
Accession NZ_CP030283
Organism Escherichia coli strain E308

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag DQW89_RS26135 Protein ID WP_000220561.1
Coordinates 48519..48800 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag DQW89_RS26140 Protein ID WP_000121743.1
Coordinates 48790..49041 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DQW89_RS26105 43996..44232 - 237 Protein_62 hypothetical protein -
DQW89_RS26110 44253..45047 - 795 WP_000572405.1 aminoglycoside O-phosphotransferase APH(3')-IIa -
DQW89_RS26115 45076..46506 - 1431 Protein_64 IS4-like element IS50R family transposase -
DQW89_RS26120 46626..46814 + 189 WP_000957857.1 hypothetical protein -
DQW89_RS26125 46824..48022 + 1199 Protein_66 IS91 family transposase -
DQW89_RS27955 48188..48280 + 93 Protein_67 IS6 family transposase -
DQW89_RS26135 48519..48800 - 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DQW89_RS26140 48790..49041 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
DQW89_RS26150 50276..51114 + 839 Protein_70 RepB family plasmid replication initiator protein -
DQW89_RS26155 51135..51977 + 843 WP_000435064.1 replication initiation protein -
DQW89_RS26160 51981..52427 + 447 WP_001074384.1 hypothetical protein -
DQW89_RS26165 52495..52917 + 423 WP_179292036.1 DNA distortion polypeptide 3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-55 / blaTEM-1B / fosA3 / oqxA / oqxB / aph(3')-IIa / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR - 1..113645 113645
- flank IS/Tn - - 53403..54602 1199


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T107477 WP_000220561.1 NZ_CP030283:c48800-48519 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T107477 NZ_CP030283:c48800-48519 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT107477 WP_000121743.1 NZ_CP030283:c49041-48790 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT107477 NZ_CP030283:c49041-48790 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References