Detailed information of TA system TA107472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 103457..104004
• Replicon: plasmid pLKSZ01
• Accession: NZ_CP030282
• Organism: Escherichia coli strain E308

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: DQW89_RS25680
• Protein ID: WP_000969996.1
• Coordinates: 103457..103738 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: DQW89_RS25685
• Protein ID: WP_032181987.1
• Coordinates: 103735..104004 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQW89_RS25640	98615..99730	-	1116	WP_113454229.1	hypothetical protein	-
DQW89_RS25650	100084..100686	+	603	WP_045133169.1	proQ/FINO family protein	-
DQW89_RS25655	100742..101236	+	495	WP_001350009.1	hypothetical protein	-
DQW89_RS28115	101236..101502	+	267	WP_000627831.1	hypothetical protein	-
DQW89_RS25670	101979..102452	-	474	WP_077128838.1	hypothetical protein	-
DQW89_RS25675	102569..103417	-	849	WP_001057996.1	3'-5' exonuclease	-
DQW89_RS25680	103457..103738	-	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DQW89_RS25685	103735..104004	-	270	WP_032181987.1	hypothetical protein	Antitoxin
DQW89_RS27910	104059..104217	-	159	WP_000841008.1	hypothetical protein	-
DQW89_RS25700	104913..105944	-	1032	WP_044706701.1	replication initiation protein	-
DQW89_RS25715	106917..107180	+	264	WP_001324596.1	ash family protein	-
DQW89_RS25720	107098..107385	+	288	WP_000074849.1	conjugal transfer protein TraA	-
DQW89_RS25730	107827..108360	+	534	WP_001306770.1	transcription termination factor NusG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	afaC-VIII	1..111721	111721

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T107472 WP_000969996.1 NZ_CP030282:c103738-103457 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T107472 NZ_CP030282:c103738-103457 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAGCGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAATGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10177.47 Da
• Isoelectric Point: 6.7251

>AT107472 WP_032181987.1 NZ_CP030282:c104004-103735 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVEQQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSATAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT107472 NZ_CP030282:c104004-103735 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACAACAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCCGCTACTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: E9LLH3