Detailed information of TA system TA107466

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4275833..4276668
• Replicon: chromosome
• Accession: NZ_CP030281
• Organism: Escherichia coli strain E308

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: DQW89_RS22465
• Protein ID: WP_000854821.1
• Coordinates: 4276291..4276668 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: DQW89_RS22460
• Protein ID: WP_032216526.1
• Coordinates: 4275833..4276201 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQW89_RS22415	4270956..4271840	+	885	WP_032216515.1	50S ribosome-binding GTPase	-
DQW89_RS22420	4271959..4272636	+	678	WP_001097364.1	hypothetical protein	-
DQW89_RS22425	4272642..4272875	+	234	WP_001278282.1	DUF905 family protein	-
DQW89_RS22435	4272974..4273792	+	819	WP_053902307.1	DUF945 domain-containing protein	-
DQW89_RS22440	4273884..4274369	+	486	WP_032216512.1	antirestriction protein	-
DQW89_RS22445	4274385..4274861	+	477	WP_001186724.1	RadC family protein	-
DQW89_RS22450	4274930..4275151	+	222	WP_001601167.1	DUF987 domain-containing protein	-
DQW89_RS22455	4275170..4275814	+	645	Protein_4105	antitoxin of toxin-antitoxin stability system	-
DQW89_RS22460	4275833..4276201	+	369	WP_032216526.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DQW89_RS22465	4276291..4276668	+	378	WP_000854821.1	type IV toxin-antitoxin system toxin CbtA	Toxin
DQW89_RS22470	4276665..4276814	+	150	Protein_4108	hypothetical protein	-
DQW89_RS22475	4276890..4277087	+	198	WP_023563519.1	DUF957 domain-containing protein	-
DQW89_RS22480	4277172..4278014	+	843	WP_001280501.1	DUF4942 domain-containing protein	-
DQW89_RS22490	4278763..4280301	+	1539	WP_001187173.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T107466 WP_000854821.1 NZ_CP030281:4276291-4276668 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T107466 NZ_CP030281:4276291-4276668 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13810.69 Da
• Isoelectric Point: 6.2066

>AT107466 WP_032216526.1 NZ_CP030281:4275833-4276201 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLLCTVTPCFGARLVQEGNRLHYLVDRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT107466 NZ_CP030281:4275833-4276201 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCTCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGTCGACCGCGCCGGCATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54