Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3480765..3481383 Replicon chromosome
Accession NZ_CP030281
Organism Escherichia coli strain E308

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag DQW89_RS18385 Protein ID WP_001291435.1
Coordinates 3481165..3481383 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag DQW89_RS18380 Protein ID WP_000344800.1
Coordinates 3480765..3481139 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DQW89_RS18370 3475854..3477047 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
DQW89_RS18375 3477070..3480219 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
DQW89_RS18380 3480765..3481139 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
DQW89_RS18385 3481165..3481383 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
DQW89_RS18390 3481555..3482106 + 552 WP_000102564.1 maltose O-acetyltransferase -
DQW89_RS18395 3482222..3482692 + 471 WP_000136192.1 YlaC family protein -
DQW89_RS18400 3482856..3484406 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
DQW89_RS18405 3484448..3484801 - 354 WP_000878140.1 DUF1428 domain-containing protein -
DQW89_RS18415 3485180..3485491 + 312 WP_000409911.1 MGMT family protein -
DQW89_RS18420 3485522..3486094 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T107464 WP_001291435.1 NZ_CP030281:3481165-3481383 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T107464 NZ_CP030281:3481165-3481383 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT107464 WP_000344800.1 NZ_CP030281:3480765-3481139 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT107464 NZ_CP030281:3480765-3481139 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References