Detailed information of TA system TA107463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2468633..2469271
• Replicon: chromosome
• Accession: NZ_CP030281
• Organism: Escherichia coli strain E308

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: DQW89_RS13035
• Protein ID: WP_000813794.1
• Coordinates: 2469095..2469271 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: DQW89_RS13030
• Protein ID: WP_001270286.1
• Coordinates: 2468633..2469049 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQW89_RS13010	2463785..2464726	-	942	WP_001251313.1	ABC transporter permease	-
DQW89_RS13015	2464727..2465740	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
DQW89_RS13020	2465758..2466903	-	1146	WP_072778914.1	ABC transporter substrate-binding protein	-
DQW89_RS13025	2467148..2468554	-	1407	WP_000760585.1	PLP-dependent aminotransferase family protein	-
DQW89_RS13030	2468633..2469049	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
DQW89_RS13035	2469095..2469271	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
DQW89_RS13040	2469493..2469723	+	231	WP_000494244.1	YncJ family protein	-
DQW89_RS13045	2469815..2471776	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
DQW89_RS13050	2471849..2472385	-	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
DQW89_RS13055	2472438..2473652	+	1215	WP_071597387.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2473692..2474957	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T107463 WP_000813794.1 NZ_CP030281:c2469271-2469095 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T107463 NZ_CP030281:c2469271-2469095 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT107463 WP_001270286.1 NZ_CP030281:c2469049-2468633 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT107463 NZ_CP030281:c2469049-2468633 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB