Detailed information of TA system TA107448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 557534..558087
• Replicon: chromosome
• Accession: NZ_CP030278
• Organism: Ethanoligenens harbinense strain W1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: DRA42_RS02720
• Protein ID: WP_083803592.1
• Coordinates: 557534..557761 (-)
• Length: 76 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E6U9C3
• Locus tag: DRA42_RS02725
• Protein ID: WP_013484486.1
• Coordinates: 557812..558087 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DRA42_RS02690	553187..554338	+	1152	WP_013484482.1	ABC transporter permease	-
DRA42_RS14275	554451..554816	-	366	WP_175405609.1	hypothetical protein	-
DRA42_RS02705	555174..555605	+	432	WP_013484484.1	sigma-70 family RNA polymerase sigma factor	-
DRA42_RS02710	555660..555854	+	195	WP_083803591.1	DUF3847 domain-containing protein	-
DRA42_RS02715	556049..557485	+	1437	Protein_528	MobA/MobL family protein	-
DRA42_RS02720	557534..557761	-	228	WP_083803592.1	type II toxin-antitoxin system YafQ family toxin	Toxin
DRA42_RS02725	557812..558087	-	276	WP_013484486.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
DRA42_RS02730	558552..558752	+	201	WP_013484488.1	transposon-encoded TnpW family protein	-
DRA42_RS02735	559015..559125	+	111	Protein_532	transposase	-
DRA42_RS02740	559359..560690	+	1332	WP_013484489.1	ferredoxin reductase family protein	-
DRA42_RS02745	560725..561108	+	384	WP_013484490.1	cytochrome b5	-
DRA42_RS02750	561191..561442	+	252	WP_041687065.1	SHOCT domain-containing protein	-
DRA42_RS02755	561499..562185	+	687	WP_041686605.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	542851..641602	98751

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-73)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-87)

Sequences

Toxin

Protein

• Length: 76 a.a.
• Molecular weight: 8692.04 Da
• Isoelectric Point: 8.0648

>T107448 WP_083803592.1 NZ_CP030278:c557761-557534 [Ethanoligenens harbinense]
MKRGLKMDLLDNIIRQLSKGQLLPEKNRDHLLTGNWVGYRECHIQSDWLLVYRIENGVLVLTLSRTGSHSDLFGE

Nucleotide

• Length: 228 bp

>T107448 NZ_CP030278:c557761-557534 [Ethanoligenens harbinense]
ATGAAGCGCGGGCTGAAAATGGATTTGCTGGACAATATCATCCGTCAGCTTTCCAAAGGTCAGCTTTTACCAGAGAAGAA
CCGCGATCATCTTCTGACCGGAAATTGGGTGGGATACCGGGAATGCCATATTCAAAGCGATTGGCTGCTTGTGTATCGAA
TTGAGAATGGCGTTCTGGTTTTAACGCTGTCCCGTACAGGCTCGCACAGTGATTTGTTTGGAGAGTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10138.54 Da
• Isoelectric Point: 6.2990

>AT107448 WP_013484486.1 NZ_CP030278:c558087-557812 [Ethanoligenens harbinense]
MANTTNFSVRMDSDIKKQCEALYSELGVNLTTAINVFLRQSLRSGGFPFDVTLNTPNAVTLAAMQEAERLAKDPNAKRFS
DVEEALRELKK

Nucleotide

• Length: 276 bp

>AT107448 NZ_CP030278:c558087-557812 [Ethanoligenens harbinense]
ATGGCCAATACGACAAATTTCAGCGTCCGCATGGACAGCGATATTAAAAAACAGTGCGAGGCGCTTTACAGTGAACTCGG
TGTGAATCTCACGACGGCTATCAACGTGTTTTTGCGGCAATCGCTTCGATCTGGCGGTTTCCCGTTCGACGTGACGCTCA
ACACGCCGAACGCCGTTACCCTCGCCGCCATGCAGGAAGCGGAGCGGCTTGCTAAAGACCCCAACGCAAAACGCTTCAGC
GATGTGGAGGAAGCGTTGAGGGAACTGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A386XKC6