Detailed information of TA system TA107446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 6395..7032
• Replicon: plasmid pC
• Accession: NZ_CP030275
• Organism: Rhodobacter sphaeroides 2.4.1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DQL45_RS21540
• Protein ID: WP_011331362.1
• Coordinates: 6625..7032 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q3IV80
• Locus tag: DQL45_RS21535
• Protein ID: WP_011331361.1
• Coordinates: 6395..6625 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQL45_RS21515	2372..3259	-	888	WP_011331357.1	viral aspartic protease	-
DQL45_RS21520	3822..4247	-	426	WP_011331358.1	helix-turn-helix transcriptional regulator	-
DQL45_RS21525	4535..4960	+	426	WP_011331359.1	hypothetical protein	-
DQL45_RS21530	5173..6006	+	834	WP_017140476.1	hypothetical protein	-
DQL45_RS21535	6395..6625	+	231	WP_011331361.1	antitoxin	Antitoxin
DQL45_RS21540	6625..7032	+	408	WP_011331362.1	tRNA(fMet)-specific endonuclease VapC	Toxin
DQL45_RS21545	7354..7512	+	159	WP_017140474.1	SDR family oxidoreductase	-
DQL45_RS21550	7521..10145	+	2625	WP_017140473.1	cation-transporting P-type ATPase	-
DQL45_RS21555	10177..10344	+	168	Protein_11	Hsp20/alpha crystallin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fcl / gmd	1..106892	106892

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14801.00 Da
• Isoelectric Point: 6.8714

>T107446 WP_011331362.1 NZ_CP030275:6625-7032 [Rhodobacter sphaeroides 2.4.1]
MLRYMLDTNILIYTIRNRPGEVRAKFEAHHGTMCISAATAMELIYGAQKSQAVERNVAAVEGLLARLDILDLDLAAAQHA
GEIRAQLAQAGTPIGPFDVMIAGHARSKGLVLVSNNTREFERVDGLRLENWVQSS

Nucleotide

• Length: 408 bp

>T107446 NZ_CP030275:6625-7032 [Rhodobacter sphaeroides 2.4.1]
ATGCTGCGCTACATGCTCGACACGAACATCCTGATCTACACGATCAGGAACCGGCCCGGCGAGGTCAGGGCAAAGTTCGA
GGCCCATCACGGGACCATGTGCATCTCGGCCGCAACCGCGATGGAACTGATATACGGTGCGCAGAAATCGCAGGCCGTCG
AGCGCAACGTGGCGGCGGTCGAGGGACTCCTCGCGCGCCTCGACATTCTGGATCTCGATCTGGCGGCTGCCCAGCATGCC
GGGGAGATCCGGGCGCAGTTGGCGCAGGCAGGGACGCCCATCGGGCCGTTCGACGTGATGATCGCGGGACACGCGCGATC
TAAGGGCCTGGTCCTCGTGAGCAACAATACGCGGGAATTCGAACGCGTCGACGGCCTGAGACTGGAGAACTGGGTTCAGT
CGTCCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8777.77 Da
• Isoelectric Point: 4.5600

>AT107446 WP_011331361.1 NZ_CP030275:6395-6625 [Rhodobacter sphaeroides 2.4.1]
VDRGSVFMNNRTQAIRLPKSVALPEGTRSVDIVRLGRAWLIVPSQDSWAQWFDEVSSSEDFMERRKQPDADERDDL

Nucleotide

• Length: 231 bp

>AT107446 NZ_CP030275:6395-6625 [Rhodobacter sphaeroides 2.4.1]
GTGGATCGGGGATCGGTCTTCATGAACAACCGGACACAGGCCATCCGCCTGCCAAAATCAGTTGCCTTGCCGGAAGGCAC
GCGGAGCGTCGACATCGTCCGGCTGGGCAGAGCCTGGCTCATCGTGCCGAGCCAGGACAGCTGGGCCCAATGGTTCGATG
AGGTCTCTTCGAGTGAAGACTTCATGGAGCGCCGGAAGCAACCGGACGCCGATGAGCGCGACGATCTCTGA

Structures

Antitoxin

• AlphaFold DB: Q3IV80