Detailed information of TA system TA107445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1410053..1410603
• Replicon: chromosome
• Accession: NZ_CP030271
• Organism: Rhodobacter sphaeroides 2.4.1

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q3J2R5
• Locus tag: DQL45_RS07030
• Protein ID: WP_002719915.1
• Coordinates: 1410053..1410301 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q3J2R4
• Locus tag: DQL45_RS07035
• Protein ID: WP_011337726.1
• Coordinates: 1410298..1410603 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQL45_RS22935	1405467..1405760	+	294	WP_147175732.1	hypothetical protein	-
DQL45_RS06995	1405757..1406545	+	789	WP_017140199.1	hypothetical protein	-
DQL45_RS07000	1406560..1407213	+	654	WP_011337721.1	head decoration protein	-
DQL45_RS07005	1407258..1407731	+	474	WP_017140200.1	hypothetical protein	-
DQL45_RS07010	1407724..1408125	+	402	WP_011337723.1	hypothetical protein	-
DQL45_RS07015	1408118..1408570	+	453	WP_002719913.1	hypothetical protein	-
DQL45_RS07020	1408567..1408800	+	234	WP_011337724.1	tail protein X	-
DQL45_RS07030	1410053..1410301	+	249	WP_002719915.1	BrnT family toxin	Toxin
DQL45_RS07035	1410298..1410603	+	306	WP_011337726.1	BrnA antitoxin family protein	Antitoxin
DQL45_RS07040	1411133..1411405	+	273	WP_011337727.1	hypothetical protein	-
DQL45_RS07045	1411501..1412313	-	813	WP_023003613.1	hypothetical protein	-
DQL45_RS23200	1412429..1412569	-	141	WP_002719918.1	hypothetical protein	-
DQL45_RS07050	1412697..1412990	+	294	WP_011337728.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1401005..1412313	11308

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (23-97)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 8950.40 Da
• Isoelectric Point: 6.4624

>T107445 WP_002719915.1 NZ_CP030271:1410053-1410301 [Rhodobacter sphaeroides 2.4.1]
MLVIVWDEPKRRSNLAKHGLDFADLDEEFFLSSVVVPASAGRHMAIGRLEDGTIAVVFAVLGAEGVSIISMRPASRKERS
LL

Nucleotide

• Length: 249 bp

>T107445 NZ_CP030271:1410053-1410301 [Rhodobacter sphaeroides 2.4.1]
ATGTTGGTGATTGTATGGGATGAGCCAAAGCGGCGCAGCAACCTGGCGAAGCATGGGCTGGACTTCGCGGACCTAGACGA
GGAGTTCTTCCTGTCGTCGGTTGTCGTGCCAGCAAGCGCAGGCCGTCACATGGCGATCGGGCGCCTTGAGGATGGAACCA
TTGCCGTGGTCTTCGCGGTTCTGGGGGCCGAGGGCGTTTCGATCATCTCTATGCGCCCTGCGAGCCGAAAGGAGAGGAGC
CTTCTATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11061.61 Da
• Isoelectric Point: 10.5767

>AT107445 WP_011337726.1 NZ_CP030271:1410298-1410603 [Rhodobacter sphaeroides 2.4.1]
MTKKRQLDTEETRIQRMIASDPDAPEATDAQLAQARPFTEAFPVLAEAMRRSAGGRPKLDAPKMSVSLRLDQDVVAHFKA
GGPGWQSRMNRALREAVGLSG

Nucleotide

• Length: 306 bp

>AT107445 NZ_CP030271:1410298-1410603 [Rhodobacter sphaeroides 2.4.1]
ATGACGAAGAAGCGTCAACTCGACACGGAAGAGACCCGCATCCAGCGGATGATCGCGAGCGACCCGGATGCTCCCGAAGC
GACCGACGCGCAGCTTGCGCAGGCCCGACCCTTCACTGAGGCGTTTCCTGTGCTGGCCGAGGCCATGCGGCGCAGCGCAG
GCGGTCGCCCGAAGCTGGACGCGCCGAAAATGTCGGTTTCACTTCGCCTCGATCAGGACGTGGTGGCGCACTTCAAGGCA
GGCGGCCCCGGTTGGCAATCTCGGATGAACCGCGCCCTGCGGGAGGCGGTCGGCCTCTCCGGCTAG

Structures

Toxin

• AlphaFold DB: Q3J2R5

Antitoxin

• AlphaFold DB: Q3J2R4