Detailed information of TA system TA107444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 154478..155148
• Replicon: plasmid pSC7-vir
• Accession: NZ_CP030270
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain SC-7

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: DQQ08_RS28150
• Protein ID: WP_004213072.1
• Coordinates: 154478..154921 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: DQQ08_RS28155
• Protein ID: WP_004213073.1
• Coordinates: 154918..155148 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQQ08_RS28105	149889..150164	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
DQQ08_RS28110	150227..150718	+	492	WP_011251327.1	DM13 domain-containing protein	-
DQQ08_RS28115	150767..151687	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
DQQ08_RS28125	151778..152184	+	407	Protein_158	GAF domain-containing protein	-
DQQ08_RS29870	152700..153334	-	635	Protein_159	response regulator	-
DQQ08_RS28140	153751..154044	+	294	Protein_160	transposase	-
DQQ08_RS28145	154048..154464	+	417	Protein_161	restriction endonuclease	-
DQQ08_RS28150	154478..154921	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
DQQ08_RS28155	154918..155148	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DQQ08_RS28160	155756..156889	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
DQQ08_RS28165	156905..157198	+	294	WP_004213076.1	hypothetical protein	-
DQQ08_RS28170	157188..157394	-	207	WP_004213077.1	hypothetical protein	-
DQQ08_RS28175	157746..158036	+	291	WP_004213078.1	hypothetical protein	-
DQQ08_RS28180	158026..158925	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	sul1 / qacE / ant(3'')-Ia / cmlA1 / aadA2	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA / pla	1..236809	236809

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T107444 WP_004213072.1 NZ_CP030270:c154921-154478 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T107444 NZ_CP030270:c154921-154478 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT107444 WP_004213073.1 NZ_CP030270:c155148-154918 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT107444 NZ_CP030270:c155148-154918 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620