Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3415051..3415676 Replicon chromosome
Accession NZ_CP030269
Organism Klebsiella pneumoniae subsp. pneumoniae strain SC-7

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag DQQ08_RS17695 Protein ID WP_012737091.1
Coordinates 3415051..3415434 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag DQQ08_RS17700 Protein ID WP_004150355.1
Coordinates 3415434..3415676 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DQQ08_RS17680 3412417..3413319 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
DQQ08_RS17685 3413316..3413951 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
DQQ08_RS17690 3413948..3414877 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
DQQ08_RS17695 3415051..3415434 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
DQQ08_RS17700 3415434..3415676 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
DQQ08_RS17705 3415881..3416798 + 918 WP_012737090.1 alpha/beta hydrolase -
DQQ08_RS17710 3416812..3417753 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
DQQ08_RS17715 3417798..3418235 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
DQQ08_RS17720 3418232..3419092 - 861 WP_004146232.1 virulence factor BrkB family protein -
DQQ08_RS17725 3419086..3419685 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T107438 WP_012737091.1 NZ_CP030269:c3415434-3415051 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T107438 NZ_CP030269:c3415434-3415051 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT107438 WP_004150355.1 NZ_CP030269:c3415676-3415434 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT107438 NZ_CP030269:c3415676-3415434 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References