Detailed information of TA system TA107423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 2013696..2014279
• Replicon: chromosome
• Accession: NZ_CP030265
• Organism: Skermanella pratensis strain W17

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DPR14_RS09105
• Protein ID: WP_158044856.1
• Coordinates: 2013932..2014279 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DPR14_RS09100
• Protein ID: WP_158044855.1
• Coordinates: 2013696..2013935 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DPR14_RS09085	2010020..2010709	+	690	WP_158044853.1	helix-turn-helix domain-containing protein	-
DPR14_RS09090	2010726..2011085	-	360	WP_158044854.1	ATP-binding protein	-
DPR14_RS09095	2011082..2012599	-	1518	WP_192499364.1	IS21 family transposase	-
DPR14_RS09100	2013696..2013935	+	240	WP_158044855.1	antitoxin MazE family protein	Antitoxin
DPR14_RS09105	2013932..2014279	+	348	WP_158044856.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DPR14_RS09110	2014325..2015583	-	1259	Protein_1819	IS3 family transposase	-
DPR14_RS09115	2015763..2015909	+	147	WP_158044857.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2003328..2057274	53946
	-	inside	IScluster/Tn	-	-	2006196..2015583	9387

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-113)

Antitoxin

• Antitoxin MazE-like (13-71)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12409.38 Da
• Isoelectric Point: 10.3460

>T107423 WP_158044856.1 NZ_CP030265:2013932-2014279 [Skermanella pratensis]
VNVVPAAKRGEVWTVSGGPDYARKPRPAVILQDDRFDTLRSITVCGFTSTGTDAPLFRLTVDPSETNGLAKPSEIMVDKI
MTVPRAKLGQRVGRLSDADMVRLNRAVIVFLGLAG

Nucleotide

• Length: 348 bp

>T107423 NZ_CP030265:2013932-2014279 [Skermanella pratensis]
GTGAACGTCGTCCCTGCGGCCAAACGGGGTGAGGTCTGGACGGTGTCCGGCGGCCCCGATTACGCCAGGAAGCCGAGACC
GGCGGTCATCCTCCAGGACGACCGTTTCGACACCCTACGATCCATCACCGTCTGCGGCTTCACCTCCACAGGCACCGATG
CCCCGCTATTCCGCCTCACGGTCGATCCCAGCGAGACCAATGGACTGGCGAAGCCCAGCGAGATCATGGTCGATAAGATC
ATGACGGTGCCCCGGGCGAAACTTGGTCAGCGAGTCGGTCGCCTGAGCGACGCCGACATGGTGCGGCTCAACCGGGCGGT
GATCGTTTTCCTCGGGTTGGCCGGCTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9093.27 Da
• Isoelectric Point: 6.4705

>AT107423 WP_158044855.1 NZ_CP030265:2013696-2013935 [Skermanella pratensis]
MTATPKSSREKTRAKVRTHREKLRAQGLRPIQIWVPDVRSPEFAAEAHRQSLLVSQSPQEAEDMAWVEAVSLPIWDEEE

Nucleotide

• Length: 240 bp

>AT107423 NZ_CP030265:2013696-2013935 [Skermanella pratensis]
ATGACAGCAACCCCAAAGTCATCCAGGGAGAAGACCAGGGCCAAGGTCCGCACCCACCGGGAAAAGCTGCGAGCACAGGG
TTTGCGGCCGATCCAGATATGGGTTCCGGATGTCCGGTCGCCGGAGTTCGCCGCCGAGGCACACCGGCAGTCGCTCCTCG
TCAGCCAGAGCCCTCAAGAAGCCGAGGATATGGCATGGGTCGAAGCCGTGTCGCTGCCGATCTGGGATGAGGAAGAGTGA