Detailed information of TA system TA107418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 597671..598341
• Replicon: plasmid AB
• Accession: NZ_CP030264
• Organism: Ensifer adhaerens strain Corn53

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DQW09_RS30300
• Protein ID: WP_060529946.1
• Coordinates: 597671..598090 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: DQW09_RS30305
• Protein ID: WP_060529947.1
• Coordinates: 598087..598341 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQW09_RS30275	592770..593660	-	891	WP_060529942.1	DMT family transporter	-
DQW09_RS30280	593650..594621	-	972	WP_060529943.1	amino acid ABC transporter permease	-
DQW09_RS30285	594651..595931	-	1281	WP_060530009.1	FAD-binding oxidoreductase	-
DQW09_RS30290	595991..596818	-	828	WP_060529944.1	transporter substrate-binding domain-containing protein	-
DQW09_RS30295	597000..597473	-	474	WP_060529945.1	hypothetical protein	-
DQW09_RS30300	597671..598090	-	420	WP_060529946.1	type II toxin-antitoxin system VapC family toxin	Toxin
DQW09_RS30305	598087..598341	-	255	WP_060529947.1	hypothetical protein	Antitoxin
DQW09_RS30310	598772..599653	-	882	WP_060529948.1	ABC transporter permease	-
DQW09_RS30315	599646..600599	-	954	WP_060529949.1	ABC transporter permease	-
DQW09_RS30320	600662..602272	-	1611	WP_060529950.1	peptide ABC transporter substrate-binding protein	-
DQW09_RS30325	602348..603235	-	888	WP_060529951.1	glyoxylate/hydroxypyruvate reductase A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..1583018	1583018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14725.81 Da
• Isoelectric Point: 4.8311

>T107418 WP_060529946.1 NZ_CP030264:c598090-597671 [Ensifer adhaerens]
MIVLDTNVVSEAMKPAPDLAVRNWLNDQVAETLYLSSVTLAELLFGIAALPEGRRKKALADTLDGLLELFDDRVLSFDTA
AARHYAGLAATARAGGKGFPTPDGYIAAIAASKGFTIATRDTSPFEAAGVPVVNPWNHL

Nucleotide

• Length: 420 bp

>T107418 NZ_CP030264:c598090-597671 [Ensifer adhaerens]
ATGATCGTCCTGGACACGAATGTCGTCTCGGAGGCGATGAAGCCCGCCCCGGATTTGGCTGTGCGCAATTGGTTGAATGA
CCAGGTAGCCGAAACCTTGTATCTGTCCAGTGTAACGCTGGCGGAACTGCTGTTTGGCATCGCTGCGCTCCCGGAGGGCC
GGCGCAAAAAAGCGTTGGCCGACACGCTTGATGGCCTGCTCGAACTGTTCGACGATCGCGTGTTGTCATTCGACACTGCG
GCGGCTCGGCACTATGCCGGCCTTGCGGCCACCGCGCGAGCAGGGGGGAAGGGATTCCCGACACCTGATGGATATATTGC
GGCTATTGCAGCCTCCAAGGGATTTACAATCGCGACACGCGATACAAGCCCCTTTGAGGCGGCAGGAGTCCCTGTCGTCA
ATCCTTGGAACCACCTATAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9581.88 Da
• Isoelectric Point: 5.7425

>AT107418 WP_060529947.1 NZ_CP030264:c598341-598087 [Ensifer adhaerens]
MAILTVRNVPDEVHRALRVRAANHGRSTEAEVREILERAVKPEQRIRIGDALAELSGRIGLTNDDFEVFDQMRDKAPAEP
MRFE

Nucleotide

• Length: 255 bp

>AT107418 NZ_CP030264:c598341-598087 [Ensifer adhaerens]
ATGGCTATCCTGACAGTACGAAATGTACCTGATGAAGTGCACCGCGCTTTGCGCGTCCGGGCGGCAAATCACGGCCGTAG
CACAGAGGCGGAAGTCCGCGAGATTCTGGAACGTGCGGTAAAGCCCGAACAGCGTATTCGCATCGGGGATGCCTTGGCTG
AGTTGAGCGGGCGGATTGGCTTGACCAATGATGATTTTGAAGTGTTCGACCAGATGCGAGACAAAGCGCCGGCAGAGCCA
ATGAGGTTTGAATGA