Detailed information of TA system TA107416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TacT2-ataR/DUF1778(antitoxin)
• Location: 976775..977581
• Replicon: plasmid AA
• Accession: NZ_CP030263
• Organism: Ensifer adhaerens strain Corn53

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: A0A1H0D3K9
• Locus tag: DQW09_RS24030
• Protein ID: WP_034798314.1
• Coordinates: 977078..977581 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: DQW09_RS24025
• Protein ID: WP_034798315.1
• Coordinates: 976775..977074 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQW09_RS23985	971870..972676	+	807	Protein_860	aspartate-alanine antiporter	-
DQW09_RS23990	972896..973183	+	288	Protein_861	decarboxylase	-
DQW09_RS23995	973521..974777	+	1257	WP_082572872.1	DUF2092 domain-containing protein	-
DQW09_RS24000	974774..974986	+	213	WP_034798320.1	hypothetical protein	-
DQW09_RS24005	975225..975596	-	372	WP_034798318.1	hypothetical protein	-
DQW09_RS24010	975642..975902	-	261	WP_051659426.1	hypothetical protein	-
DQW09_RS24015	976135..976368	+	234	WP_034798317.1	hypothetical protein	-
DQW09_RS24020	976422..976616	-	195	Protein_867	transposase	-
DQW09_RS24025	976775..977074	+	300	WP_034798315.1	DUF1778 domain-containing protein	Antitoxin
DQW09_RS24030	977078..977581	+	504	WP_034798314.1	GNAT family N-acetyltransferase	Toxin
DQW09_RS24035	977700..977933	-	234	Protein_870	hypothetical protein	-
DQW09_RS24045	978802..978945	-	144	WP_156553069.1	hypothetical protein	-
DQW09_RS35205	978942..979952	-	1011	WP_192441885.1	PAS domain S-box protein	-
DQW09_RS35210	979953..980642	-	690	Protein_873	GAF domain-containing protein	-
DQW09_RS35215	980637..981023	-	387	Protein_874	PAS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	katA	1..1790007	1790007

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (12-87)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 17521.15 Da
• Isoelectric Point: 7.8065

>T107416 WP_034798314.1 NZ_CP030263:977078-977581 [Ensifer adhaerens]
VALSTPEPLTAAHDVSEFSCGKPTLDHWLKTRALSNQQKGFTAILVVHEAGRVVGYYGLAPTAVVPSVMPRSIRTGQPPN
PVPCLLLGQLATDTGWAGQGIGTGLVKHALQRCVQAAGLVGGRALMVNAVDDEAAGFWQRRGFLPSKDDPLVLFRSIADV
AASLAEA

Nucleotide

• Length: 504 bp

>T107416 NZ_CP030263:977078-977581 [Ensifer adhaerens]
GTGGCGTTATCCACTCCCGAACCGCTTACCGCCGCGCATGATGTGTCTGAATTCTCTTGCGGCAAGCCGACGCTCGATCA
CTGGCTGAAGACGCGCGCGCTTTCCAATCAGCAGAAGGGCTTCACCGCCATTTTGGTCGTCCATGAGGCGGGGCGCGTCG
TCGGCTATTATGGTCTGGCGCCGACGGCCGTGGTGCCGTCGGTCATGCCGCGCTCGATTCGCACAGGCCAGCCGCCCAAT
CCGGTTCCGTGCCTTCTTCTTGGCCAGCTTGCCACCGATACCGGATGGGCGGGGCAGGGGATTGGGACGGGTCTCGTCAA
ACACGCGCTGCAGCGTTGCGTCCAGGCGGCCGGTCTGGTGGGCGGGCGAGCGCTGATGGTCAATGCCGTGGATGATGAAG
CTGCCGGTTTCTGGCAACGGCGTGGCTTCTTGCCGTCGAAGGACGATCCACTTGTCCTCTTCCGCTCGATTGCCGATGTC
GCGGCGTCGCTGGCCGAGGCGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10953.58 Da
• Isoelectric Point: 4.9273

>AT107416 WP_034798315.1 NZ_CP030263:976775-977074 [Ensifer adhaerens]
MATTAERKEHPISMRLPEADVAMIDRAASLRGRSRTDFVRDAAVRAAEEVVMEQSLIRMSPEGFDAFMEVLAAPAAPAPE
MVELAKRPAPWEAGYEPKR

Nucleotide

• Length: 300 bp

>AT107416 NZ_CP030263:976775-977074 [Ensifer adhaerens]
ATGGCCACCACAGCGGAACGCAAGGAACACCCCATCTCGATGCGACTGCCGGAAGCGGATGTCGCGATGATCGATCGTGC
GGCGTCGCTGCGCGGCCGCTCGCGCACCGACTTCGTGCGCGATGCCGCCGTGCGCGCGGCCGAGGAGGTCGTGATGGAGC
AAAGCCTGATCCGTATGAGCCCGGAAGGCTTCGATGCGTTCATGGAGGTTCTCGCCGCCCCGGCAGCGCCTGCTCCCGAA
ATGGTCGAGTTGGCGAAACGGCCCGCACCATGGGAAGCCGGCTACGAGCCGAAGCGGTAA

Structures

Toxin

• AlphaFold DB: A0A1H0D3K9