Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5468426..5469051 Replicon chromosome
Accession NZ_CP030173
Organism Klebsiella variicola strain 13450

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag DQB70_RS27265 Protein ID WP_008807903.1
Coordinates 5468426..5468809 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag DQB70_RS27270 Protein ID WP_004150355.1
Coordinates 5468809..5469051 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DQB70_RS27250 5465793..5466695 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
DQB70_RS27255 5466692..5467327 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
DQB70_RS27260 5467324..5468253 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
DQB70_RS27265 5468426..5468809 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
DQB70_RS27270 5468809..5469051 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
DQB70_RS27275 5469256..5470173 + 918 WP_112293758.1 alpha/beta hydrolase -
DQB70_RS27280 5470187..5471128 - 942 WP_022064599.1 fatty acid biosynthesis protein FabY -
DQB70_RS27285 5471173..5471610 - 438 WP_012543288.1 D-tyrosyl-tRNA(Tyr) deacylase -
DQB70_RS27290 5471607..5472467 - 861 WP_008807907.1 virulence factor BrkB family protein -
DQB70_RS27295 5472461..5473060 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T107312 WP_008807903.1 NZ_CP030173:c5468809-5468426 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T107312 NZ_CP030173:c5468809-5468426 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT107312 WP_004150355.1 NZ_CP030173:c5469051-5468809 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT107312 NZ_CP030173:c5469051-5468809 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References