Detailed information of TA system TA107309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4852621..4853197
• Replicon: chromosome
• Accession: NZ_CP030173
• Organism: Klebsiella variicola strain 13450

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: DQB70_RS24230
• Protein ID: WP_012542979.1
• Coordinates: 4852910..4853197 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: DQB70_RS24225
• Protein ID: WP_022065028.1
• Coordinates: 4852621..4852923 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQB70_RS24200	4848030..4849094	-	1065	WP_008807596.1	DUF2955 domain-containing protein	-
DQB70_RS24205	4849084..4850151	-	1068	WP_022065029.1	HlyD family secretion protein	-
DQB70_RS24210	4850158..4850622	-	465	WP_008807598.1	MarR family transcriptional regulator	-
DQB70_RS24215	4850788..4850952	-	165	WP_008807599.1	DUF1127 domain-containing protein	-
DQB70_RS24220	4851130..4852542	+	1413	WP_008807600.1	PLP-dependent aminotransferase family protein	-
DQB70_RS24225	4852621..4852923	-	303	WP_022065028.1	BrnA antitoxin family protein	Antitoxin
DQB70_RS24230	4852910..4853197	-	288	WP_012542979.1	BrnT family toxin	Toxin
DQB70_RS24235	4853365..4853784	-	420	WP_048271457.1	FosA family fosfomycin resistance glutathione transferase	-
DQB70_RS24240	4853778..4854686	-	909	WP_016160219.1	LysR family transcriptional regulator	-
DQB70_RS24245	4854773..4855555	+	783	WP_012542981.1	NAD(P)H-dependent oxidoreductase	-
DQB70_RS24250	4855697..4856281	+	585	WP_022065974.1	TetR/AcrR family transcriptional regulator	-
DQB70_RS24255	4856303..4857106	+	804	WP_022065975.1	winged helix-turn-helix domain-containing protein	-
DQB70_RS24260	4857103..4857621	+	519	WP_004182811.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (38-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11245.77 Da
• Isoelectric Point: 8.6574

>T107309 WP_012542979.1 NZ_CP030173:c4853197-4852910 [Klebsiella variicola]
MPMEFEWDANKALSNLRKHGVRFEEAVLVFDDPRHLSRQERFENGEYRWQTIGLVHGILVILVAHSVRFESGTEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T107309 NZ_CP030173:c4853197-4852910 [Klebsiella variicola]
ATGCCAATGGAGTTTGAGTGGGATGCAAACAAGGCATTAAGTAATCTACGAAAACATGGAGTCCGGTTTGAAGAAGCGGT
ATTAGTCTTTGACGATCCGCGGCACCTTTCCCGCCAGGAGCGGTTTGAGAATGGTGAATATCGCTGGCAGACCATCGGTC
TTGTGCATGGGATCCTTGTTATCCTGGTGGCGCATTCGGTCCGTTTTGAAAGTGGAACTGAGGTTATTCGTATTATCAGC
GCCCGGAAGGCGGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11320.98 Da
• Isoelectric Point: 10.3129

>AT107309 WP_022065028.1 NZ_CP030173:c4852923-4852621 [Klebsiella variicola]
MSMVKHKRGNTAALTAKRDAELKALASKADEDIDYSDIPATDDAQWSDAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT107309 NZ_CP030173:c4852923-4852621 [Klebsiella variicola]
ATGAGCATGGTTAAACATAAACGCGGCAATACGGCGGCGCTTACCGCTAAGCGTGATGCCGAACTCAAAGCGCTAGCGAG
CAAAGCCGATGAGGATATCGATTACAGCGATATCCCCGCTACAGACGATGCGCAATGGTCAGATGCCGTTCGCGGCAAAT
TTTTTCGACCGCTAAAAACACAGGCTTCTGTGCGCATTGATGCCGATGTCATGGAGTGGCTCAAACGTCCAGGAAAAGGC
TATCAAACTCGACTGAATGCGATTTTAAGAGAAGCGATGATTCGCGATCTTAATAAGAAATAG