Detailed information of TA system TA107294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 352259..352845
• Replicon: chromosome
• Accession: NZ_CP030173
• Organism: Klebsiella variicola strain 13450

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: DQB70_RS01715
• Protein ID: WP_012967118.1
• Coordinates: 352477..352845 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: DQB70_RS01710
• Protein ID: WP_004174006.1
• Coordinates: 352259..352480 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQB70_RS01690	348408..349334	+	927	WP_012540389.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
DQB70_RS01695	349331..350608	+	1278	WP_008806971.1	branched chain amino acid ABC transporter permease LivM	-
DQB70_RS01700	350605..351372	+	768	WP_008806972.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
DQB70_RS01705	351374..352087	+	714	WP_022066270.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
DQB70_RS01710	352259..352480	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DQB70_RS01715	352477..352845	+	369	WP_012967118.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
DQB70_RS01720	353137..354453	+	1317	WP_022066271.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
DQB70_RS01725	354560..355447	+	888	WP_012967120.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
DQB70_RS01730	355444..356289	+	846	WP_008806976.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
DQB70_RS01735	356291..357361	+	1071	WP_022066272.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	350605..358098	7493

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (26-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13584.98 Da
• Isoelectric Point: 8.6410

>T107294 WP_012967118.1 NZ_CP030173:352477-352845 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGMTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T107294 NZ_CP030173:352477-352845 [Klebsiella variicola]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGACCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTATGACAGACGTGT
GGCGACTCGCTGCGATGCACCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCCGACTTCGTCGGCATGACCGTCGAGGCTGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT107294 WP_004174006.1 NZ_CP030173:352259-352480 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT107294 NZ_CP030173:352259-352480 [Klebsiella variicola]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCGGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A5E5YJY7