Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3905057..3905676 Replicon chromosome
Accession NZ_CP030172
Organism Klebsiella pneumoniae subsp. pneumoniae strain 12208

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag DQB68_RS19445 Protein ID WP_002892050.1
Coordinates 3905458..3905676 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag DQB68_RS19440 Protein ID WP_002892066.1
Coordinates 3905057..3905431 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DQB68_RS19430 3900209..3901402 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
DQB68_RS19435 3901425..3904571 + 3147 WP_004147373.1 multidrug efflux RND transporter permease subunit -
DQB68_RS19440 3905057..3905431 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
DQB68_RS19445 3905458..3905676 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
DQB68_RS19450 3905835..3906401 + 567 WP_002892030.1 maltose O-acetyltransferase -
DQB68_RS26720 3906373..3906513 - 141 WP_004147370.1 hypothetical protein -
DQB68_RS19455 3906534..3907004 + 471 WP_002892026.1 YlaC family protein -
DQB68_RS19460 3906979..3908430 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
DQB68_RS19465 3908531..3909229 + 699 WP_040220001.1 GNAT family N-acetyltransferase -
DQB68_RS19470 3909226..3909366 - 141 WP_040219999.1 type B 50S ribosomal protein L36 -
DQB68_RS19475 3909366..3909629 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T107287 WP_002892050.1 NZ_CP030172:3905458-3905676 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T107287 NZ_CP030172:3905458-3905676 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT107287 WP_002892066.1 NZ_CP030172:3905057-3905431 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT107287 NZ_CP030172:3905057-3905431 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References