Detailed information of TA system TA107262

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 114780..115321
• Replicon: plasmid unnamed
• Accession: NZ_CP030165
• Organism: Xanthomonas citri pv. punicae strain LMG7439

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: XapA_RS23790
• Protein ID: WP_015462614.1
• Coordinates: 114780..115106 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H8FMA7
• Locus tag: XapA_RS23795
• Protein ID: WP_003491150.1
• Coordinates: 115103..115321 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XapA_RS23780	110148..111722	+	1575	WP_005931760.1	type IV secretion system DNA-binding domain-containing protein	-
XapA_RS23785	111736..114711	+	2976	WP_005931763.1	conjugative relaxase	-
XapA_RS23790	114780..115106	-	327	WP_015462614.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
XapA_RS23795	115103..115321	-	219	WP_003491150.1	antitoxin MazE family protein	Antitoxin
XapA_RS23800	115458..115793	-	336	WP_005931770.1	hypothetical protein	-
XapA_RS23805	115824..116183	-	360	WP_033480402.1	hypothetical protein	-
XapA_RS23810	116358..117236	-	879	WP_005931773.1	transglycosylase SLT domain-containing protein	-
XapA_RS23815	117247..118269	-	1023	WP_005931776.1	P-type DNA transfer ATPase VirB11	-
XapA_RS23820	118280..119500	-	1221	WP_005931779.1	TrbI/VirB10 family protein	-
XapA_RS23825	119502..120284	-	783	WP_011052985.1	P-type conjugative transfer protein VirB9	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..157583	157583

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11676.70 Da
• Isoelectric Point: 9.2550

>T107262 WP_015462614.1 NZ_CP030165:c115106-114780 [Xanthomonas citri pv. punicae]
MMRGDFVTIAMQGDFGKPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T107262 NZ_CP030165:c115106-114780 [Xanthomonas citri pv. punicae]
ATGATGCGGGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAAAACCAAGGCCCGCTCTGGTGATCCAGGCCGA
CCAGTTTGACGCACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTCGTTGCTGCGCCGCTGCTGCGTATTACCG
TTCATCCAAGTACCGACAATGGTTTGCAGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
GTAGGTCGAGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8418.54 Da
• Isoelectric Point: 5.6599

>AT107262 WP_003491150.1 NZ_CP030165:c115321-115103 [Xanthomonas citri pv. punicae]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT107262 NZ_CP030165:c115321-115103 [Xanthomonas citri pv. punicae]
ATGGCACACGTCAATTCACGAGTTCAAAAGCACCGCGACGCCCTGCGCATGGCAGGGCTGCGTCCGGTGCAAATTTGGGT
GCCGGACACACGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTGTCGTCTTGCTGCACAAGCGGACATGGCGGACA
CCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCTGGACGGAATGA

Structures

Antitoxin

• AlphaFold DB: H8FMA7