Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 8776..9241 Replicon plasmid pJSMCR1_5
Accession NZ_CP030157
Organism Escherichia coli strain JSMCR1

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag JSMCR1_RS25860 Protein ID WP_001230707.1
Coordinates 8990..9241 (-) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag JSMCR1_RS25855 Protein ID WP_000801440.1
Coordinates 8776..9003 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSMCR1_RS25810 (JSMCR1_p658) 4097..4330 - 234 WP_000121161.1 EexN family lipoprotein -
JSMCR1_RS25815 (JSMCR1_p659) 4335..4601 - 267 WP_000160395.1 hypothetical protein -
JSMCR1_RS25820 (JSMCR1_p660) 4678..5907 - 1230 WP_001694183.1 DNA relaxase/nickase TaxC -
JSMCR1_RS25825 (JSMCR1_p661) 5919..6473 - 555 WP_256625400.1 DNA distortion polypeptide 1 -
JSMCR1_RS25830 (JSMCR1_p664) 6783..7130 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
JSMCR1_RS25835 (JSMCR1_p665) 7127..7375 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
JSMCR1_RS25840 (JSMCR1_p667) 7682..7987 - 306 WP_072182957.1 hypothetical protein -
JSMCR1_RS25845 (JSMCR1_p668) 8055..8327 - 273 WP_001272373.1 hypothetical protein -
JSMCR1_RS25850 (JSMCR1_p669) 8454..8774 - 321 WP_224693477.1 molecular chaperone DnaJ -
JSMCR1_RS25855 (JSMCR1_p670) 8776..9003 - 228 WP_000801440.1 hypothetical protein Antitoxin
JSMCR1_RS25860 (JSMCR1_p671) 8990..9241 - 252 WP_001230707.1 hypothetical protein Toxin
JSMCR1_RS25865 (JSMCR1_p672) 9238..9780 - 543 WP_001083906.1 hypothetical protein -
JSMCR1_RS25870 (JSMCR1_p673) 9885..10400 - 516 WP_001025395.1 J domain-containing protein -
JSMCR1_RS25875 (JSMCR1_p677) 11900..12682 + 783 WP_256625401.1 replication initiation protein -
JSMCR1_RS25880 (JSMCR1_p678) 12695..13099 + 405 WP_000654311.1 hypothetical protein -
JSMCR1_RS25885 (JSMCR1_p680) 13234..13761 + 528 WP_000577044.1 DUF2726 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..29589 29589


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(29-132)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T107255 WP_001230707.1 NZ_CP030157:c9241-8990 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T107255 NZ_CP030157:c9241-8990 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATCACATTGTATGATGCCGTGCTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT107255 WP_000801440.1 NZ_CP030157:c9003-8776 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT107255 NZ_CP030157:c9003-8776 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References