Detailed information of TA system TA107254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 6783..7375
• Replicon: plasmid pJSMCR1_5
• Accession: NZ_CP030157
• Organism: Escherichia coli strain JSMCR1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V0AGQ2
• Locus tag: JSMCR1_RS25835
• Protein ID: WP_000520549.1
• Coordinates: 7127..7375 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: JSMCR1_RS25830
• Protein ID: WP_000681613.1
• Coordinates: 6783..7130 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSMCR1_RS25800 (JSMCR1_p655)	2781..3107	-	327	WP_000008647.1	TrbC/VirB2 family protein	-
JSMCR1_RS25805 (JSMCR1_p656)	3121..3741	-	621	WP_053911938.1	lytic transglycosylase domain-containing protein	-
JSMCR1_RS25810 (JSMCR1_p658)	4097..4330	-	234	WP_000121161.1	EexN family lipoprotein	-
JSMCR1_RS25815 (JSMCR1_p659)	4335..4601	-	267	WP_000160395.1	hypothetical protein	-
JSMCR1_RS25820 (JSMCR1_p660)	4678..5907	-	1230	WP_001694183.1	DNA relaxase/nickase TaxC	-
JSMCR1_RS25825 (JSMCR1_p661)	5919..6473	-	555	WP_256625400.1	DNA distortion polypeptide 1	-
JSMCR1_RS25830 (JSMCR1_p664)	6783..7130	-	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JSMCR1_RS25835 (JSMCR1_p665)	7127..7375	-	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	Toxin
JSMCR1_RS25840 (JSMCR1_p667)	7682..7987	-	306	WP_072182957.1	hypothetical protein	-
JSMCR1_RS25845 (JSMCR1_p668)	8055..8327	-	273	WP_001272373.1	hypothetical protein	-
JSMCR1_RS25850 (JSMCR1_p669)	8454..8774	-	321	WP_224693477.1	molecular chaperone DnaJ	-
JSMCR1_RS25855 (JSMCR1_p670)	8776..9003	-	228	WP_000801440.1	hypothetical protein	-
JSMCR1_RS25860 (JSMCR1_p671)	8990..9241	-	252	WP_001230707.1	hypothetical protein	-
JSMCR1_RS25865 (JSMCR1_p672)	9238..9780	-	543	WP_001083906.1	hypothetical protein	-
JSMCR1_RS25870 (JSMCR1_p673)	9885..10400	-	516	WP_001025395.1	J domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..29589	29589

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9422.08 Da
• Isoelectric Point: 10.4800

>T107254 WP_000520549.1 NZ_CP030157:c7375-7127 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T107254 NZ_CP030157:c7375-7127 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT107254 WP_000681613.1 NZ_CP030157:c7130-6783 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT107254 NZ_CP030157:c7130-6783 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGGAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCACTGAACGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A829CIX3

Antitoxin

• AlphaFold DB: A0A829CLC1