Detailed information of TA system TA107251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 81964..82511
• Replicon: plasmid pJSMCR1_3
• Accession: NZ_CP030155
• Organism: Escherichia coli strain JSMCR1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G7QEJ9
• Locus tag: JSMCR1_RS25250
• Protein ID: WP_001384452.1
• Coordinates: 81964..82242 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: JSMCR1_RS25255
• Protein ID: WP_032181987.1
• Coordinates: 82242..82511 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSMCR1_RS25220 (77561)	77561..77956	+	396	WP_256625388.1	hypothetical protein	-
JSMCR1_RS25225 (78045)	78045..78233	+	189	WP_000957857.1	hypothetical protein	-
JSMCR1_RS25230 (78243)	78243..79442	+	1200	WP_000948429.1	IS91 family transposase	-
JSMCR1_RS25235 (79743)	79743..80009	+	267	WP_000627831.1	hypothetical protein	-
JSMCR1_RS25240 (80486)	80486..80959	-	474	WP_077128838.1	hypothetical protein	-
JSMCR1_RS25245 (81076)	81076..81924	-	849	WP_001057996.1	3'-5' exonuclease	-
JSMCR1_RS25250 (81964)	81964..82242	-	279	WP_001384452.1	type II toxin-antitoxin system toxin YacB	Toxin
JSMCR1_RS25255 (82242)	82242..82511	-	270	WP_032181987.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
JSMCR1_RS25260 (83420)	83420..84451	-	1032	WP_044706701.1	plasmid replication initiator RepA	-
JSMCR1_RS25265 (85457)	85457..85687	+	231	WP_228772074.1	ash family protein	-
JSMCR1_RS25270 (85605)	85605..85892	+	288	WP_000074849.1	conjugal transfer protein TraA	-
JSMCR1_RS25275 (86334)	86334..86867	+	534	WP_001306770.1	transcription termination/antitermination NusG family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	afaC-I	1..109689	109689
	-	flank	IS/Tn	-	-	78243..79442	1199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10762.38 Da
• Isoelectric Point: 8.4615

>T107251 WP_001384452.1 NZ_CP030155:c82242-81964 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T107251 NZ_CP030155:c82242-81964 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAGCGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAATGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10177.47 Da
• Isoelectric Point: 6.7251

>AT107251 WP_032181987.1 NZ_CP030155:c82511-82242 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVEQQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSATAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT107251 NZ_CP030155:c82511-82242 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACAACAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCCGCTACTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A829CL36