Detailed information of TA system TA107245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 58198..58799
• Replicon: plasmid pJSMCR1_2
• Accession: NZ_CP030154
• Organism: Escherichia coli strain JSMCR1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A649Z4R3
• Locus tag: JSMCR1_RS24375
• Protein ID: WP_072647225.1
• Coordinates: 58419..58799 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: JSMCR1_RS24370
• Protein ID: WP_001190712.1
• Coordinates: 58198..58419 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSMCR1_RS24360 (JSMCR1_p298)	55194..56462	-	1269	WP_072647224.1	restriction endonuclease subunit S	-
JSMCR1_RS24365 (JSMCR1_p299)	56459..58015	-	1557	WP_072647227.1	type I restriction-modification system subunit M	-
JSMCR1_RS24370 (JSMCR1_p300)	58198..58419	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JSMCR1_RS24375 (JSMCR1_p301)	58419..58799	+	381	WP_072647225.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
JSMCR1_RS24380 (JSMCR1_p302)	58804..58983	+	180	WP_000113018.1	hypothetical protein	-
JSMCR1_RS24385 (JSMCR1_p303)	59011..60054	+	1044	WP_072647226.1	DUF968 domain-containing protein	-
JSMCR1_RS24390 (JSMCR1_p304)	60178..61146	-	969	WP_000654811.1	IS5 family transposase	-
JSMCR1_RS24395	61315..61434	+	120	WP_072647269.1	ash family protein	-
JSMCR1_RS24400 (JSMCR1_p306)	61453..61674	+	222	WP_001361625.1	host cell division inhibitor Icd-like protein	-
JSMCR1_RS24405	61983..62333	+	351	Protein_59	ORF6N domain-containing protein	-
JSMCR1_RS24410	62454..62786	+	333	WP_224205061.1	phage antirepressor KilAC domain-containing protein	-
JSMCR1_RS24415 (JSMCR1_p308)	62819..63670	-	852	WP_000611664.1	phage repressor protein C1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(A) / qnrS1 / ARR-3 / cmlA1 / blaOXA-10 / ant(3'')-Ia / dfrA14 / floR	-	1..134064	134064

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13572.34 Da
• Isoelectric Point: 5.6343

>T107245 WP_072647225.1 NZ_CP030154:58419-58799 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELAVLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T107245 NZ_CP030154:58419-58799 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTGATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGTCCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT107245 WP_001190712.1 NZ_CP030154:58198-58419 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT107245 NZ_CP030154:58198-58419 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A649Z4R3

Antitoxin

• AlphaFold DB: A0A829CJB6