Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 4741..5384 Replicon plasmid pJSMCR1_1
Accession NZ_CP030153
Organism Escherichia coli strain JSMCR1

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag JSMCR1_RS23240 Protein ID WP_001044768.1
Coordinates 4968..5384 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag JSMCR1_RS23235 Protein ID WP_001261287.1
Coordinates 4741..4971 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSMCR1_RS23215 (117) 117..851 + 735 WP_256625369.1 hypothetical protein -
JSMCR1_RS23220 (1027) 1027..1809 - 783 WP_256625370.1 IS21-like element IS100kyp family helper ATPase IstB -
JSMCR1_RS23225 (1806) 1806..2828 - 1023 WP_000255944.1 IS21-like element IS100 family transposase -
JSMCR1_RS23230 (2895) 2895..4433 - 1539 WP_256625371.1 AAA family ATPase -
JSMCR1_RS23235 (4741) 4741..4971 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JSMCR1_RS23240 (4968) 4968..5384 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
JSMCR1_RS23245 (5546) 5546..7684 - 2139 WP_000350635.1 AAA family ATPase -
JSMCR1_RS23250 (8038) 8038..8295 + 258 WP_000343085.1 hypothetical protein -
JSMCR1_RS23255 (8295) 8295..8885 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / fosA3 / aac(3)-IId / blaCTX-M-65 / sitABCD iroB / iroC / iroD / iroE / iroN 1..152677 152677


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T107241 WP_001044768.1 NZ_CP030153:4968-5384 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T107241 NZ_CP030153:4968-5384 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT107241 WP_001261287.1 NZ_CP030153:4741-4971 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT107241 NZ_CP030153:4741-4971 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References