Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4313233..4313730 Replicon chromosome
Accession NZ_CP030152
Organism Escherichia coli strain JSMCR1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JSMCR1_RS20935 Protein ID WP_001173343.1
Coordinates 4313233..4313406 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A2U9LI53
Locus tag JSMCR1_RS20940 Protein ID WP_071597404.1
Coordinates 4313434..4313730 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSMCR1_RS20925 (4309898) 4309898..4311355 + 1458 WP_072778207.1 dipeptide/tripeptide permease DtpC -
JSMCR1_RS20930 (4311592) 4311592..4313109 + 1518 WP_001295074.1 lysine--tRNA ligase -
JSMCR1_RS20935 (4313233) 4313233..4313406 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JSMCR1_RS20940 (4313434) 4313434..4313730 - 297 WP_071597404.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JSMCR1_RS20945 (4313957) 4313957..4314229 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
JSMCR1_RS20950 (4314241) 4314241..4314471 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
JSMCR1_RS20955 (4314652) 4314652..4316283 + 1632 WP_001565376.1 sensor histidine kinase -
JSMCR1_RS20960 (4316280) 4316280..4316999 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(6-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T107240 WP_001173343.1 NZ_CP030152:c4313406-4313233 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T107240 NZ_CP030152:c4313406-4313233 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11495.99 Da        Isoelectric Point: 4.1705

>AT107240 WP_071597404.1 NZ_CP030152:c4313730-4313434 [Escherichia coli]
MEGKNRFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT107240 NZ_CP030152:c4313730-4313434 [Escherichia coli]
ATGGAAGGTAAAAACAGGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A2U9LI53

References