Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3515241..3515859 Replicon chromosome
Accession NZ_CP030152
Organism Escherichia coli strain JSMCR1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JSMCR1_RS17030 Protein ID WP_001291435.1
Coordinates 3515641..3515859 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JSMCR1_RS17025 Protein ID WP_000344800.1
Coordinates 3515241..3515615 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSMCR1_RS17015 (3510330) 3510330..3511523 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JSMCR1_RS17020 (3511546) 3511546..3514695 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JSMCR1_RS17025 (3515241) 3515241..3515615 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JSMCR1_RS17030 (3515641) 3515641..3515859 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JSMCR1_RS17035 (3516031) 3516031..3516321 + 291 Protein_3330 maltose O-acetyltransferase -
JSMCR1_RS17040 (3516371) 3516371..3517068 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
JSMCR1_RS17045 (3517084) 3517084..3517359 + 276 Protein_3332 maltose O-acetyltransferase -
JSMCR1_RS17050 (3517475) 3517475..3517945 + 471 WP_000136192.1 YlaC family protein -
JSMCR1_RS17055 (3518109) 3518109..3519659 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JSMCR1_RS17060 (3519701) 3519701..3520054 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JSMCR1_RS17070 (3520433) 3520433..3520744 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T107237 WP_001291435.1 NZ_CP030152:3515641-3515859 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T107237 NZ_CP030152:3515641-3515859 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT107237 WP_000344800.1 NZ_CP030152:3515241-3515615 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT107237 NZ_CP030152:3515241-3515615 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References