Detailed information of TA system TA107236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2479532..2480170
• Replicon: chromosome
• Accession: NZ_CP030152
• Organism: Escherichia coli strain JSMCR1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: JSMCR1_RS11935
• Protein ID: WP_000813794.1
• Coordinates: 2479994..2480170 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JSMCR1_RS11930
• Protein ID: WP_001270286.1
• Coordinates: 2479532..2479948 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSMCR1_RS11910 (2474684)	2474684..2475625	-	942	WP_001251313.1	ABC transporter permease	-
JSMCR1_RS11915 (2475626)	2475626..2476639	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
JSMCR1_RS11920 (2476657)	2476657..2477802	-	1146	WP_072778914.1	ABC transporter substrate-binding protein	-
JSMCR1_RS11925 (2478047)	2478047..2479453	-	1407	WP_000760585.1	PLP-dependent aminotransferase family protein	-
JSMCR1_RS11930 (2479532)	2479532..2479948	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JSMCR1_RS11935 (2479994)	2479994..2480170	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JSMCR1_RS11940 (2480392)	2480392..2480622	+	231	WP_000494244.1	YncJ family protein	-
JSMCR1_RS11945 (2480714)	2480714..2482675	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JSMCR1_RS11950 (2482748)	2482748..2483284	-	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
JSMCR1_RS11955 (2483376)	2483376..2484551	+	1176	WP_001236251.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2484591..2485856	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T107236 WP_000813794.1 NZ_CP030152:c2480170-2479994 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T107236 NZ_CP030152:c2480170-2479994 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT107236 WP_001270286.1 NZ_CP030152:c2479948-2479532 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT107236 NZ_CP030152:c2479948-2479532 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB