Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 905652..906306 Replicon chromosome
Accession NZ_CP030152
Organism Escherichia coli strain JSMCR1

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag JSMCR1_RS04380 Protein ID WP_000244781.1
Coordinates 905899..906306 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JSMCR1_RS04375 Protein ID WP_000354046.1
Coordinates 905652..905918 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSMCR1_RS04355 (901740) 901740..903173 - 1434 WP_001394742.1 6-phospho-beta-glucosidase BglA -
JSMCR1_RS04360 (903218) 903218..903529 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
JSMCR1_RS04365 (903693) 903693..904352 + 660 WP_000250274.1 hemolysin III family protein -
JSMCR1_RS04370 (904429) 904429..905409 - 981 WP_000886087.1 tRNA-modifying protein YgfZ -
JSMCR1_RS04375 (905652) 905652..905918 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JSMCR1_RS04380 (905899) 905899..906306 + 408 WP_000244781.1 protein YgfX Toxin
JSMCR1_RS04385 (906346) 906346..906867 - 522 WP_001055874.1 flavodoxin FldB -
JSMCR1_RS04390 (906979) 906979..907875 + 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
JSMCR1_RS04395 (907900) 907900..908610 + 711 WP_000715222.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JSMCR1_RS04400 (908616) 908616..910349 + 1734 WP_110843999.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T107224 WP_000244781.1 NZ_CP030152:905899-906306 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T107224 NZ_CP030152:905899-906306 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT107224 WP_000354046.1 NZ_CP030152:905652-905918 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT107224 NZ_CP030152:905652-905918 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References