Detailed information of TA system TA107220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1292526..1293443
• Replicon: chromosome
• Accession: NZ_CP030150
• Organism: Bacillus velezensis strain DSYZ

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: BVDSYZ_RS06710
• Protein ID: WP_007407256.1
• Coordinates: 1292697..1293443 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: BVDSYZ_RS06705
• Protein ID: WP_003154807.1
• Coordinates: 1292526..1292696 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVDSYZ_RS06670	1287749..1289380	+	1632	WP_015417294.1	pyocin knob domain-containing protein	-
BVDSYZ_RS06675	1289393..1289764	+	372	WP_015417295.1	YomQ/XkdW protein, phage-like element PBSX	-
BVDSYZ_RS06680	1289769..1289966	+	198	WP_012117366.1	XkdX family protein	-
BVDSYZ_RS06685	1290023..1290784	+	762	WP_033575307.1	hypothetical protein	-
BVDSYZ_RS06690	1290836..1291099	+	264	WP_003154815.1	hemolysin XhlA family protein	-
BVDSYZ_RS06695	1291113..1291376	+	264	WP_003154813.1	phage holin	-
BVDSYZ_RS06700	1291390..1292268	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
BVDSYZ_RS06705	1292526..1292696	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
BVDSYZ_RS06710	1292697..1293443	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BVDSYZ_RS06715	1293547..1294545	-	999	WP_003154805.1	inorganic phosphate transporter	-
BVDSYZ_RS06720	1294558..1295175	-	618	WP_003154804.1	DUF47 domain-containing protein	-
BVDSYZ_RS06725	1295461..1296777	-	1317	WP_007610842.1	amino acid permease	-
BVDSYZ_RS06730	1297100..1298050	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T107220 WP_007407256.1 NZ_CP030150:c1293443-1292697 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T107220 NZ_CP030150:c1293443-1292697 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT107220 WP_003154807.1 NZ_CP030150:c1292696-1292526 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT107220 NZ_CP030150:c1292696-1292526 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14