Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 24920..25563 Replicon plasmid pKPC2_L111
Accession NZ_CP030134
Organism Klebsiella pneumoniae strain 160111

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag DL890_RS29400 Protein ID WP_001044770.1
Coordinates 24920..25336 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag DL890_RS29405 Protein ID WP_001261282.1
Coordinates 25333..25563 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DL890_RS29380 21023..21295 - 273 Protein_27 transposase -
DL890_RS29385 21324..22021 - 698 Protein_28 IS1 family transposase -
DL890_RS29390 22277..23299 - 1023 WP_000361404.1 helicase UvrD -
DL890_RS29395 23284..24846 - 1563 WP_004206609.1 AAA family ATPase -
DL890_RS29400 24920..25336 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
DL890_RS29405 25333..25563 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
DL890_RS29410 25520..25981 + 462 WP_014343465.1 hypothetical protein -
DL890_RS29415 26142..27086 + 945 WP_011977810.1 hypothetical protein -
DL890_RS29420 27123..27515 + 393 WP_011977811.1 hypothetical protein -
DL890_RS29425 27573..28094 + 522 WP_013214008.1 hypothetical protein -
DL890_RS29430 28140..28343 + 204 WP_011977813.1 hypothetical protein -
DL890_RS29435 28373..29377 + 1005 WP_011977814.1 hypothetical protein -
DL890_RS29440 29561..30340 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-65 / blaKPC-2 - 1..60307 60307


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T107163 WP_001044770.1 NZ_CP030134:c25336-24920 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T107163 NZ_CP030134:c25336-24920 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT107163 WP_001261282.1 NZ_CP030134:c25563-25333 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT107163 NZ_CP030134:c25563-25333 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References