Detailed information of TA system TA107154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1042132..1042700
• Replicon: plasmid unnamed1
• Accession: NZ_CP030127
• Organism: Indioceanicola profundi strain SCSIO 08040

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DOL89_RS20745
• Protein ID: WP_119681233.1
• Coordinates: 1042371..1042700 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DOL89_RS20740
• Protein ID: WP_119681232.1
• Coordinates: 1042132..1042374 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DOL89_RS20720	1037599..1038282	+	684	WP_119681229.1	UdgX family uracil-DNA binding protein	-
DOL89_RS20725	1038550..1039341	+	792	WP_119681230.1	methyltransferase domain-containing protein	-
DOL89_RS20730	1039268..1040224	-	957	WP_119681393.1	EamA family transporter	-
DOL89_RS20735	1040464..1041888	-	1425	WP_119681231.1	3-isopropylmalate dehydratase large subunit	-
DOL89_RS20740	1042132..1042374	+	243	WP_119681232.1	antitoxin MazE family protein	Antitoxin
DOL89_RS20745	1042371..1042700	+	330	WP_119681233.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DOL89_RS20750	1042697..1042996	-	300	WP_162937758.1	RNA-binding S4 domain-containing protein	-
DOL89_RS20755	1043062..1046313	-	3252	WP_119681235.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd	1..1184037	1184037

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-104)

Antitoxin

• Antitoxin MazE-like (18-79)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11922.76 Da
• Isoelectric Point: 9.1642

>T107154 WP_119681233.1 NZ_CP030127:1042371-1042700 [Indioceanicola profundi]
MRRGDIVTVAAKGAYTAKPRPAVIIQSDRFDETGSVTLCLISRTELSTPLIRLDIQPSAQNGLAEPSWIMVDKIATVPRK
DVRGTNGRVTDEDMVRLNRALTVFLGIAD

Nucleotide

• Length: 330 bp

>T107154 NZ_CP030127:1042371-1042700 [Indioceanicola profundi]
ATGAGGCGGGGTGACATCGTCACCGTGGCTGCCAAAGGCGCTTACACCGCGAAGCCCCGGCCCGCCGTCATCATCCAGTC
CGACCGGTTCGATGAAACCGGCTCCGTTACCTTGTGCCTGATCAGCCGCACCGAGCTGTCCACGCCGCTCATCCGGCTGG
ATATCCAGCCATCCGCACAGAACGGCCTCGCCGAGCCGTCCTGGATCATGGTCGACAAGATCGCGACCGTGCCGCGCAAG
GATGTACGCGGCACGAACGGGCGTGTCACGGACGAGGACATGGTGCGCCTGAACCGCGCCCTGACCGTCTTCCTCGGCAT
TGCGGATTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9311.43 Da
• Isoelectric Point: 10.5662

>AT107154 WP_119681232.1 NZ_CP030127:1042132-1042374 [Indioceanicola profundi]
MSRSKSIRSETAREAGRRKTQLYRDRLRAQGLRPIQLWVPDVRSPRFQEEAKRQSRIVAVSPEEGEINDFLADVQDWGGE

Nucleotide

• Length: 243 bp

>AT107154 NZ_CP030127:1042132-1042374 [Indioceanicola profundi]
ATGTCCAGATCCAAATCCATCAGGTCCGAGACGGCGCGTGAGGCAGGGCGGCGCAAGACGCAGCTTTATCGTGACCGGCT
GCGGGCACAGGGGCTGCGACCCATCCAGCTCTGGGTGCCGGACGTGCGCTCCCCCCGCTTCCAGGAAGAGGCCAAGCGTC
AGTCGCGCATTGTCGCCGTCTCGCCCGAGGAAGGCGAGATAAACGACTTTCTCGCCGACGTTCAGGACTGGGGCGGGGAA
TGA