Detailed information of TA system TA107139

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 515750..516403
• Replicon: chromosome
• Accession: NZ_CP030117
• Organism: Brevibacillus brevis strain DZQ7

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: J2PLP3
• Locus tag: AB432_RS02900
• Protein ID: WP_005830205.1
• Coordinates: 516053..516403 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: J2GZH3
• Locus tag: AB432_RS02895
• Protein ID: WP_007721089.1
• Coordinates: 515750..516049 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB432_RS02870	510841..511878	+	1038	WP_048036222.1	outer membrane lipoprotein-sorting protein	-
AB432_RS02880	512608..513690	+	1083	WP_048036223.1	hypothetical protein	-
AB432_RS02885	513875..514315	+	441	WP_048036224.1	hypothetical protein	-
AB432_RS02890	514318..515520	+	1203	WP_048036225.1	alanine racemase	-
AB432_RS02895	515750..516049	+	300	WP_007721089.1	CopG family ribbon-helix-helix protein	Antitoxin
AB432_RS02900	516053..516403	+	351	WP_005830205.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AB432_RS02905	516607..517035	+	429	WP_048036226.1	anti-sigma regulatory factor	-
AB432_RS02910	517041..518042	+	1002	WP_048036227.1	PP2C family protein-serine/threonine phosphatase	-
AB432_RS02915	518066..518392	+	327	WP_048036228.1	anti-sigma factor antagonist	-
AB432_RS02920	518392..518886	+	495	WP_048036229.1	anti-sigma B factor RsbW	-
AB432_RS02925	518855..519643	+	789	WP_048036230.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (15-52)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12917.86 Da
• Isoelectric Point: 4.8435

>T107139 WP_005830205.1 NZ_CP030117:516053-516403 [Brevibacillus brevis]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVNESLQISLGLIDF

Nucleotide

• Length: 351 bp

>T107139 NZ_CP030117:516053-516403 [Brevibacillus brevis]
GTGATTGTCAAACGTGGCGATGTGTTTTTCGCGGACCTGTCCCCGGTCGTTGGCTCTGAGCAAGGAGGCGTACGGCCTGT
TTTAGTCATTCAAAACGACATTGGAAATCGATTTAGTCCGACGGTCATCGTCGCAGCCATTACGGCGCAGATTCAAAAGG
CGAAGTTGCCTACGCATGTGGAAATTGACGCAAAAACTTATGGATTTGATCGTGACTCCGTTATTCTGTTGGAACAAATA
CGAACCATTGACAAACAACGTCTAACTGATAAAATAACGCATCTCGATGACGAGATGATGGATCGGGTTAATGAATCCTT
GCAAATCAGTTTAGGACTCATTGATTTTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11309.19 Da
• Isoelectric Point: 10.2101

>AT107139 WP_007721089.1 NZ_CP030117:515750-516049 [Brevibacillus brevis]
MVGGGFVLSKSNTKRIMISLPQHLLQEVDGVIQKEKSNRSEFIRQAMHLYLKERKKRTIRETMQKGYMEMAKINLNMASE
AFGAEEEADHTLVRLVSGV

Nucleotide

• Length: 300 bp

>AT107139 NZ_CP030117:515750-516049 [Brevibacillus brevis]
ATGGTTGGAGGTGGATTTGTCTTGTCGAAGTCAAACACGAAACGCATCATGATCAGCTTGCCACAACACTTGCTTCAGGA
AGTGGATGGCGTTATTCAAAAGGAAAAATCCAATCGCAGCGAGTTTATTCGTCAAGCGATGCATTTGTATTTGAAAGAGC
GAAAAAAACGCACAATCCGTGAGACCATGCAAAAAGGGTACATGGAGATGGCGAAGATCAATCTGAATATGGCATCGGAG
GCTTTTGGCGCTGAAGAAGAGGCTGACCATACTCTTGTCCGCTTAGTTAGCGGGGTGTGA

Structures

Toxin

• AlphaFold DB: J2PLP3

Antitoxin

• AlphaFold DB: J2GZH3