Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 16975..17500 Replicon plasmid pNMEC-O75A
Accession NZ_CP030112
Organism Escherichia coli strain MCJCHV-1

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag DPQ29_RS25575 Protein ID WP_001159868.1
Coordinates 17195..17500 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag DPQ29_RS25570 Protein ID WP_000813634.1
Coordinates 16975..17193 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DPQ29_RS25540 12368..12784 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
DPQ29_RS25545 12781..13011 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
DPQ29_RS25550 13276..13776 + 501 WP_001773886.1 hypothetical protein -
DPQ29_RS25555 13789..14562 + 774 WP_000905949.1 hypothetical protein -
DPQ29_RS25560 14729..15862 + 1134 WP_001542067.1 DUF3800 domain-containing protein -
DPQ29_RS25565 15896..16408 - 513 WP_000151784.1 hypothetical protein -
DPQ29_RS25570 16975..17193 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
DPQ29_RS25575 17195..17500 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
DPQ29_RS25580 17501..18307 + 807 WP_000016982.1 site-specific integrase -
DPQ29_RS25585 19081..19836 + 756 WP_000852146.1 replication initiation protein RepE -
DPQ29_RS25590 20424..21590 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / aac(3)-IId senB 1..88421 88421


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T107138 WP_001159868.1 NZ_CP030112:17195-17500 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T107138 NZ_CP030112:17195-17500 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT107138 WP_000813634.1 NZ_CP030112:16975-17193 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT107138 NZ_CP030112:16975-17193 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References