Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 12368..13011 Replicon plasmid pNMEC-O75A
Accession NZ_CP030112
Organism Escherichia coli strain MCJCHV-1

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag DPQ29_RS25540 Protein ID WP_001034046.1
Coordinates 12368..12784 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag DPQ29_RS25545 Protein ID WP_001261278.1
Coordinates 12781..13011 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DPQ29_RS25525 7505..7921 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
DPQ29_RS25530 7918..8148 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
DPQ29_RS25535 8529..12323 + 3795 WP_001144732.1 hypothetical protein -
DPQ29_RS25540 12368..12784 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
DPQ29_RS25545 12781..13011 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
DPQ29_RS25550 13276..13776 + 501 WP_001773886.1 hypothetical protein -
DPQ29_RS25555 13789..14562 + 774 WP_000905949.1 hypothetical protein -
DPQ29_RS25560 14729..15862 + 1134 WP_001542067.1 DUF3800 domain-containing protein -
DPQ29_RS25565 15896..16408 - 513 WP_000151784.1 hypothetical protein -
DPQ29_RS25570 16975..17193 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
DPQ29_RS25575 17195..17500 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / aac(3)-IId senB 1..88421 88421


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T107137 WP_001034046.1 NZ_CP030112:c12784-12368 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T107137 NZ_CP030112:c12784-12368 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT107137 WP_001261278.1 NZ_CP030112:c13011-12781 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT107137 NZ_CP030112:c13011-12781 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References