107134

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4418319..4418816 Replicon chromosome
Accession NZ_CP030111
Organism Escherichia coli strain MCJCHV-1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag DPQ29_RS22915 Protein ID WP_001173343.1
Coordinates 4418319..4418492 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag DPQ29_RS22920 Protein ID WP_001312331.1
Coordinates 4418520..4418816 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DPQ29_RS22905 4414989..4416446 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
DPQ29_RS22910 4416683..4418200 + 1518 WP_000003806.1 lysine--tRNA ligase -
DPQ29_RS22915 4418319..4418492 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
DPQ29_RS22920 4418520..4418816 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
DPQ29_RS22925 4419045..4419317 - 273 WP_000405647.1 N-acetyltransferase -
DPQ29_RS22930 4419329..4419559 - 231 WP_000373293.1 (4Fe-4S)-binding protein -
DPQ29_RS22935 4419740..4421371 + 1632 WP_001216482.1 sensor histidine kinase -
DPQ29_RS22940 4421368..4422087 + 720 WP_001540474.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4362498..4418816 56318


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T107134 WP_001173343.1 NZ_CP030111:c4418492-4418319 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T107134 NZ_CP030111:c4418492-4418319 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT107134 WP_001312331.1 NZ_CP030111:c4418816-4418520 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT107134 NZ_CP030111:c4418816-4418520 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References