Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3994660..3995210 Replicon chromosome
Accession NZ_CP030111
Organism Escherichia coli strain MCJCHV-1

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag DPQ29_RS20620 Protein ID WP_001160966.1
Coordinates 3994660..3994974 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag DPQ29_RS20625 Protein ID WP_000125568.1
Coordinates 3994977..3995210 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DPQ29_RS20595 3990335..3991621 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
DPQ29_RS20600 3991621..3992610 + 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
DPQ29_RS20605 3992607..3993428 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
DPQ29_RS20610 3993431..3993808 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
DPQ29_RS20615 3993815..3994663 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
DPQ29_RS20620 3994660..3994974 - 315 WP_001160966.1 CcdB family protein Toxin
DPQ29_RS20625 3994977..3995210 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
DPQ29_RS20630 3995296..3995775 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
DPQ29_RS20635 3995967..3997829 - 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
DPQ29_RS20640 3997822..3998352 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
DPQ29_RS20645 3998460..3999791 - 1332 WP_001183207.1 MFS transporter -
DPQ29_RS20650 3999850..4000137 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T107125 WP_001160966.1 NZ_CP030111:c3994974-3994660 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T107125 NZ_CP030111:c3994974-3994660 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT107125 WP_000125568.1 NZ_CP030111:c3995210-3994977 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT107125 NZ_CP030111:c3995210-3994977 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References