Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3519975..3520593 Replicon chromosome
Accession NZ_CP030111
Organism Escherichia coli strain MCJCHV-1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag DPQ29_RS18255 Protein ID WP_001291435.1
Coordinates 3520375..3520593 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag DPQ29_RS18250 Protein ID WP_000344800.1
Coordinates 3519975..3520349 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DPQ29_RS18240 3515064..3516257 + 1194 WP_001538394.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
DPQ29_RS18245 3516280..3519429 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
DPQ29_RS18250 3519975..3520349 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
DPQ29_RS18255 3520375..3520593 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
DPQ29_RS18260 3520766..3521317 + 552 WP_000102539.1 maltose O-acetyltransferase -
DPQ29_RS18265 3521433..3521903 + 471 WP_000136192.1 YlaC family protein -
DPQ29_RS18270 3522067..3523617 + 1551 WP_001538392.1 cyclic-guanylate-specific phosphodiesterase PdeB -
DPQ29_RS18275 3523659..3524012 - 354 WP_000878140.1 DUF1428 domain-containing protein -
DPQ29_RS18285 3524391..3524702 + 312 WP_000409908.1 MGMT family protein -
DPQ29_RS18290 3524733..3525305 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T107123 WP_001291435.1 NZ_CP030111:3520375-3520593 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T107123 NZ_CP030111:3520375-3520593 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT107123 WP_000344800.1 NZ_CP030111:3519975-3520349 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT107123 NZ_CP030111:3519975-3520349 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References